LncRNA WTAPP1 promotes cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1
- PDF / 1,428,555 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 41 Downloads / 199 Views
RESEARCH ARTICLE
Open Access
LncRNA WTAPP1 promotes cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1 Li Zhang1, Chengyan Jin2, Guiyun Yang3, Bin Wang2, Peiyan Hua2 and Yan Zhang2*
Abstract Background: Long non-coding RNA (lncRNA) Wilms Tumor 1 Associated Protein Pseudogene 1 (WTAPP1) has been reported to be a critical player in the angiogenesis and migration of endothelial progenitor cells, while its involvement in cancer biology remains unknown. This study was carried out to investigate the role of WTAPP1 in non-small cell lung cancer (NSCLC). Methods: The expression of WTAPP1 and lncRNA HAND2 Antisense RNA 1 (HAND2-AS1) in plasma and tissues from NSCLC patients was detected by qRT-PCR. A 5-year follow-up study was carried out to explore the prognostic value of WTAPP1 for NSCLC. Overexpression experiments were performed to analyze the interaction between WTAPP1 and HAND2-AS1. Cell invasion and migration were evaluated by Transwell assays. Results: The expression of WTAPP1 was upregulated in NSCLC. The survival analysis showed that low plasma levels of WTAPP1 were accompanied with high survival rate. HAND2-AS1 was downregulated in NSCLC and inversely correlated with WTAPP1 across tumor tissues. Overexpression of WTAPP1 resulted in downregulation of HAND2-AS1 in NSCLC cells, while overexpression of HAND2-AS1 did not affect the expression of WTAPP1. Overexpression of WTAPP1 led to promoted, while overexpression of HAND2-AS1 resulted in inhibited invasion and migration of NSCLC cells. In addition, overexpression of HAND2-AS1 partially attenuated the effects of overexpressing WTAPP1. In addition, WTAPP1 did not affect cancer cell proliferation. Conclusion: WTAPP1 may promote cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1. Keywords: Non-small cell lung cancer, lncRNA WTAPP1, lncRNA HAND2-AS1, Survival
Background Lung cancer affected 2,093,876 new cases (11.6 of all cancer patients) and caused 1,761,007 deaths (18.4% of all cancer deaths) in 2018 alone [1]. Lung cancer is the most common cancer for both incidence and mortality in many countries of the world [2] including China [3]. Lung cancer patients diagnosed before tumor invasions * Correspondence: [email protected] 2 Department of Thoracic Surgery, The Second Hospital of Jilin University, No.218 Ziqiang Street, Changchun City, Jilin Province 130041, People’s Republic of China Full list of author information is available at the end of the article
can be treated with surgical resections, and the treatment outcomes are generally satisfactory [4]. However, most patients with lung cancer are diagnosed at late stages with the existing of tumor metastasis and radical treatment approaches are not available [5]. Analysis of human transcriptome has revealed that the majority of RNA transcripts are not related to protein synthesis [6]. Long (> 200 nt) noncoding RNAs (lncRNAs) have structural and regulatory functions. Different from messenger RNAs that are templates of protein synthesis, lncRNAs function by affect
Data Loading...
