LncRNA XIST Promotes Migration and Invasion of Papillary Thyroid Cancer Cell by Modulating MiR-101-3p/CLDN1 Axis
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LncRNA XIST Promotes Migration and Invasion of Papillary Thyroid Cancer Cell by Modulating MiR‑101‑3p/CLDN1 Axis Yong‑Liang Du1 · Yan Liang1 · Yan Cao1 · Le Liu1 · Jian Li1 · Guang‑Qing Shi1 Received: 27 February 2020 / Accepted: 25 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy in the worlds. Long non-coding RNA X-inactive specific transcript (XIST) was found to upregulate in PTC tissues and cell lines. However, the molecular mechanism underlying PTC metastasis and whether XIST plays regulatory role in PTC are still largely unknown. qRT-PCR was performed to detect the expression of lncRNA XIST and mRNAs. Western blotting was carried out to detect CLDN1, MMP2, and MMP9. Transwell assay was used to detect migration and invasion. Starbase bioinformatics prediction and luciferase assay were used to validate the relationship of miR-101-3p and XIST or CLDN1. LncRNA XIST was upregulated in PTC tissues and cells. XIST knockdown suppressed migration and invasion of PTC cells. XIST could directly bind with miR-101-3p. Overexpression of miR-101-3p suppressed migration and invasion of PTC cells. CLDN1 was the target of miR-101-3p, and overexpression of CLDN1 can reverse the inhibition of cell migration and invasion by miR-101-3p, What’s more, miR-101-3p inhibition and CLDN1 overexpression can reverse the affection of sh-XIST on migration and invasion of PTC cells inhibition. XIST promotes migration and invasion of papillary thyroid cancer cell via directly regulating miR-101-3p/CLDN1 axis, which is a novel mechanistic of XIST in the regulation of PTC. Keywords PTC · lncRNA XIST · miR-101-3p · CLDN1
Yong-Liang Du and Yan Liang co-first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1052 8-020-09985-8) contains supplementary material, which is available to authorized users. * Jian Li [email protected] * Guang‑Qing Shi [email protected] 1
Nuclear Medicine Department, The Third Xiangya Hospital of Central South University, No.137 Tongzipo Road, Yuelu District, Changsha 410013, Hunan, People’s Republic of China
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Biochemical Genetics
Abbreviations PTC Papillary thyroid carcinoma XIST X-inactive specific transcript RAI Radioactive iodine NSCLC Non-small-cell lung cancer HCC Hepatocellular carcinoma CRC Colorectal cancer CLDN1 Claudin-1 PBS Phosphate-buffered saline RPMI Roswell Park Memorial Institute qRT-PCR Quantitative real-time polymerase chain reaction MMP Matrix metalloproteinase lncRNAs Long non-coding RNAs ceRNA Competing endogenous RNA
Introduction Papillary thyroid carcinoma (PTC), the most common endocrine malignancy in the worlds, accounts for ~ 90% of all thyroid cancer (Nguyen et al. 2015). Most patients with PTC have a good prognosis by surgical removal, followed by adjuvant radioactive iodine (RAI) therapy (Rivkees et al. 2011). However, a small fraction of patients with PTC do not respo
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