Long noncoding RNA MIR99AHG promotes gastric cancer progression by inducing EMT and inhibiting apoptosis via miR577/FOXP
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PRIMARY RESEARCH
Cancer Cell International Open Access
Long noncoding RNA MIR99AHG promotes gastric cancer progression by inducing EMT and inhibiting apoptosis via miR577/FOXP1 axis Qingyang Meng1†, Xiangjun Wang2†, Tongqing Xue3, Qiangfang Zhao3, Wei Wang3 and Kun Zhao3*
Abstract Background: Long non-coding RNAs (lncRNAs) play a vital role in the genesis and development of human cancer. LncRNA MIR99AHG has been reported to be upregulated in acute myeloid leukemia (AML); however, its function in gastric cancer (GC) is still not clear. Here we were aiming to screen the prognostic lncRNA candidates and to explore the function of MIR99AHG in GC. Methods: We have preliminarily screened some candidate lncRNA biomarkers in GC tissues through analyzing microarray datasets. The expression level of MIR99AHG in GC cell lines and tissues was monitored via qPCR. Survival analysis was performed with the patients of our hospital and TCGA database cases. CCK-8 assay, trans-well assay and flow cytometry were performed to determine cell proliferation, invasion, migration and apoptosis. Meanwhile, a target of MIR99AHG was predicted and identified by luciferase reporter gene detection experiments. Results: MIR99AHG was strongly up-regulated in human GC and contributed to cancer progression. Kaplan–Meier analysis revealed that up-regulating MIR99AHG expression was positively correlated with unfavorable overall survival (P 50
28
23
Male
37
33
Female
22
27
Distal
20
24
Middle
21
23
Proximal
18
12
> 5
35
33
≤ 5
29
31
9
13
Moderately
17
20
Poorly
21
10
Undifferentiated
12
16
T1
4
15
T2
10
21
T3
20
15
T4
15
8
I–II
20
35
III–IV
39
24
Yes
39
23
No
20
36
Yes
15
7
No
44
52
Age (years)
0.732
Gender
0.554
Location
0.679
Size (cm)
0.654
Histologic differentiation Well
P-value
0.008
Invasion depth
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