Long Non-coding RNA LINC01503 Promotes Gastric Cardia Adenocarcinoma Progression via miR-133a-5p/VIM Axis and EMT Proces
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ORIGINAL ARTICLE
Long Non‑coding RNA LINC01503 Promotes Gastric Cardia Adenocarcinoma Progression via miR‑133a‑5p/VIM Axis and EMT Process Yanli Guo1 · Pingping Sun2 · Wei Guo1 · Zhiming Dong1 Received: 23 April 2020 / Accepted: 21 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background LINC01503 has been reported to act as a candidate oncogenic lncRNA in several types of human cancer. However, the functions and underlying mechanisms of LINC01503 in gastric cardia adenocarcinoma (GCA) remain unclear. Aims To investigate the roles and underlying mechanisms of LINC01503 in GCA progression. Materials and Methods Gene expressions were detected by quantitative real-time PCR (qRT-PCR). Gain-of-function assays were performed to evaluate the function of LINC01503 in gastric cancer cells. Bioinformatics analysis, luciferase reporter assay, and RIP assay were performed to identify associations among LINC01503, miR-133a-5p, and VIM. Results The expression level of LINC01503 was significantly elevated in GCA tissues and cell lines. High expression of LINC01503 was correlated with lymph node metastasis, TNM stage, and poor prognosis of GCA patients. Knockdown of LINC01503 significantly reduced proliferation, migration, and invasion ability in GC cells. LINC01503 might function as a competing endogenous RNA (ceRNA) via sponging miR-133a-5p to upregulate the expression of VIM. Furthermore, overexpression of LINC01503 promoted the progression of epithelial mesenchymal transition (EMT) in vitro. Conclusion LINC01503 serves as an oncogenic lncRNA to promote GCA progression via affecting LINC01503/miR133a-5p/VIM axis and EMT process. LINC01503 not only has a critical role in GCA progression but also provide a novel potential biomarker in predicting prognosis for GCA patients. Keywords Gastric cardia adenocarcinoma · LINC01503 · miR-133a-5p · VIM · Epithelial mesenchymal transition
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10620-020-06690-9) contains supplementary material, which is available to authorized users. * Zhiming Dong [email protected] Yanli Guo [email protected] Pingping Sun [email protected] Wei Guo [email protected] 1
Hebei Cancer Institute, The Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang 050011, Hebei, China
Beijing Shijitan Hospital, Capital Medical University, Beijing, China
2
Gastric cardia adenocarcinoma (GCA), arising in the transformation zone between the esophagus and the stomach, is the most aggressive subtype of gastric cancer (GC) with a high metastatic rate [1–3]. GCA is often identified in the advanced stage accompanied with a poor prognosis. Metastasis, particularly the distant metastasis, is the major cause for poor prognosis and low survival rate of GCA [4, 5]. Clinical data indicate that the post-surgery recurrence rate of GCA is as high as 50% [4]. China has high incidence of GCA, especially in Taihang Mountains of North
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