M-keratin nano-materials create a mineralized micro-circumstance to promote proliferation and differentiation of DPSCs
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TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES Original Research
M-keratin nano-materials create a mineralized micro-circumstance to promote proliferation and differentiation of DPSCs Wu-Ya Chen
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Xia Li1,2,3 Yingying Feng1,2,3 Siqi Lin1,2,3 Liwang Peng1,2,3 Dahong Huang1,2,3 ●
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Received: 12 November 2019 / Accepted: 3 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract As traditional root canal obturation leads to the loss of the biological activity of the tooth, it is necessary to develop a material that promotes the regeneration of dental tissue. However, this remains a challenging task. Our study aims to construct a mineralized material to support the proliferation and differentiation of dental pulp stem cells (DPSCs), and to explore a new strategy for the treatment of pulp tissue necrosis. Mineralized keratin (M-keratin), defined as keratin that has been mineralized in simulated body fluid, was first harvested to construct the root canal filling material. Characterizations indicated that new substances or components were formed on the surface of keratin particles after mineralization, and the morphology of the keratin was changed. M-keratin promoted the growth, proliferation, and differentiation of DPSCs. After cultivation with M-keratin, DPSCs exhibited more extracellular matrix proteins interacting with the culture interface, the number of these cells increased significantly, and the 3-[4,5-dimethylthiazol-2-yl-]-2,5-diphenyltetrazolium bromide values of cells in the experimental group also increased. Meanwhile, signs that the DPSCs began to differentiate into odontoblasts were observed or detected by alizarin red S staining, ELISA, RNA-Seq, and western blot. We hope that this study will contribute to the development of a new material that promotes the regeneration of dental tissue as well as providing new ideas and strategies for the treatment of dental pulp disease. Graphical Abstract
1 Introduction These authors contributed equally: Wu-Ya Chen, Xia Li Supplementary information The online version of this article (https:// doi.org/10.1007/s10856-020-06465-8) contains supplementary material, which is available to authorized users.
Dental pulp tissue necrosis or inflammation, accompanied by severe pain, adversely affects patients [1]. The main
* Wu-Ya Chen [email protected]
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* Dahong Huang [email protected]
Guangdong Engineering Research Center of Digital Stomatology, Foshan 528225, PR China
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Foshan Engineering Research Center of Stomatology, Foshan 528225, PR China
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Foshan Stomatological Hospital, School of Stomatology and Medicine, Foshan University, Foshan 528225, PR China
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Journal of Materials Science: Materials in Medicine (2020)31:124
treatment for these problems is a root canal. However, this therapy simply eliminates all tissues, lesioned or not, in the root canal and pulp cavity, replacing them with other materials instead of fundamentally repairing the pulp. The loss of root t
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