Male factor infertility impacts the rate of mosaic blastocysts in cycles of preimplantation genetic testing for aneuploi
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ASSISTED REPRODUCTION TECHNOLOGIES
Male factor infertility impacts the rate of mosaic blastocysts in cycles of preimplantation genetic testing for aneuploidy Nicoletta Tarozzi 1 & Marco Nadalini 1 & Cristina Lagalla 1 & Giovanni Coticchio 1
&
Carlotta Zacà 1 & Andrea Borini 1
Received: 18 June 2019 / Accepted: 6 September 2019 # Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Purpose In this study, we tested the hypothesis that, in PGT-A cycles, decreased semen quality is associated with increased rates of mosaic blastocysts. Methods In a retrospective analysis, three hundred and forty PGT-A cycles are divided into study groups according to semen quality. Cycles were initially divided into two groups, discerning couples with absence of male factor of infertility (non-male factor: NMF; N = 146 cycles) from couples with a male factor of infertility (MF; N = 173 cycles). Couples with severe male factor (SMF) infertility (n = 22) were assessed separately. Embryos were cultured to the blastocyst stage and chromosomally assessed by array comparative genomic hybridization (aCGH). The study did not involve specific interventions. Results The reproductive outcome of MF and NMF groups did not indicate statistically significant differences. However, while no differences were found between MF and NMF groups in terms of euploid or aneuploid blastocysts rates, a significantly higher rate of mosaic blastocysts was observed in the MF group (3.6% vs. 0.5%, respectively; P = 0.03). A similar pattern of results was observed in the SMF group when compared with those of the other PGT-A cycles taken together (no SMF). In particular, a significantly higher rate of mosaic blastocysts was observed in the SMF group (7.7% and 1.8%, respectively; P = 0.008). Conclusions The study outcome strongly suggests that compromised semen quality is associated with increased rates of mosaic blastocysts analysed in PGT-A cycles. Sperm assessment appears therefore as an important factor in the determination of embryo development and for a more precise prognostic assessment of PGT-A cases. Keywords Aneuploidy . Mosaicism . Blastocyst . Semen . Infertility
Introduction In assisted reproduction technology (ART) cycles, preimplantation genetic testing for aneuploidy (PGT-A) has long been proposed as an approach to identify and selectively use euploid embryos, in order to improve treatment efficiency and reduce time to pregnancy [1]. This strategy is justified by the fact that most of human conceptions are affected by different forms of aneuploidy, total or partial, that cause developmental failure at preimplantation or postimplantation stages or, in a Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10815-019-01584-w) contains supplementary material, which is available to authorized users. * Giovanni Coticchio [email protected] 1
9.Baby - Family and Fertility Center, Via Dante 15, 40125 Bologna, Italy
few cases, very severe pathologies in the newborns [2]. The ori
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