Mass Transfer and Rheological Characteristics in a Stirred Tank Bioreactor for Cultivation of Escherichia coli BL21
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pISSN 1226-8372 eISSN 1976-3816
RESEARCH PAPER
Mass Transfer and Rheological Characteristics in a Stirred Tank Bioreactor for Cultivation of Escherichia coli BL21 Sohail Rasool Lone, Vimal Kumar, Jeffrey R. Seay, Derek L. Englert, and Hyun Tae Hwang
Received: 29 January 2020 / Revised: 13 April 2020 / Accepted: 6 May 2020 © The Korean Society for Biotechnology and Bioengineering and Springer 2020
Abstract With the rapid growth of pharmaceutical and biotechnology industry, stirred tank bioreactors have received much attention due to simple design, easy control of operating conditions, and low operating cost. In the development of commercial processes, however, a transition from laboratory to industrial scale faces great challenges because many properties related to size change nonlinearly as a system increases. In this context, along with an understanding of fluid dynamics, application of an efficient method for scale-up is critical for designing successful industrial process. Particularly in cell cultivation processes, it is important to evaluate the oxygen transfer and viscous properties of liquid medium. In the present study, the effect of various key operating variables such as agitation rate and aeration rate, impeller diameter, and bioreactor working volume for different impellers on the volumetric mass transfer coefficient (kLa) have been investigated in a stirred tank bioreactor for cultivating Escherichia coli BL21. It was found that the kLa tends to increase with the operating variables except the bioreactor working volume. Among the tested impellers, the pitched blade was observed to be most promising because of relatively higher kLa but less shear force owing to its low power number. It was also found that the liquid medium with E. coli behaves as a Newtonian liquid. Compared to conventional designs of Rushton turbines, dislocated Rushton turbine was found to deliver higher kLa. Finally, using dimensional analysis, the kLa for different impeller configurations was correlated in Sohail Rasool Lone, Vimal Kumar Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India Sohail Rasool Lone, Jeffrey R. Seay, Derek L. Englert, Hyun Tae Hwang* Department of Chemical & Materials Engineering, University of Kentucky, 4810 Alben Barkley Drive, Paducah, Kentucky 42002, USA Tel: +1-270-534-3900; Fax: +1-270-543-3071 E-mail: [email protected]
the form of dimensionless groups, suggesting that this approach can be used for predicting kLa in different scales of stirred tank bioreactors. Keywords: stirred tank bioreactor, mass transfer coefficient, Escherichia coli, gas-liquid system, dimensional analysis
1. Introduction The design of reactors is determined by various factors such as operating conditions, physical and chemical properties of components taking part in, mass and heat transfer, control and maintenance of processes, and safety. Among the reactor types, stirred tank reactors are one of the most commonly used reactors in the industry due to their sim
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