Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embr
- PDF / 1,422,905 Bytes
- 11 Pages / 595.276 x 793.701 pts Page_size
- 29 Downloads / 188 Views
RESEARCH
Open Access
Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embryo development Chun-Hui Zhang1,2†, Wei-Ping Qian1†, Shu-Tao Qi2, Zhao-Jia Ge2, Ling-Jiang Min2,4, Xiu-Lang Zhu2, Xin Huang2, Jing-Ping Liu1, Ying-Chun Ouyang2, Yi Hou2, Heide Schatten3 and Qing-Yuan Sun2*
Abstract Background: The adverse effects of maternal diabetes on oocyte maturation and embryo development have been reported. Methods: In this study, we used time-lapse live cell imaging confocal microscopy to investigate the dynamic changes of ER and the effects of diabetes on the ER’s structural dynamics during oocyte maturation, fertilization and early embryo development. Results: We report that the ER first became remodeled into a dense ring around the developing MI spindle, and then surrounded the spindle during migration to the cortex. ER reorganization during mouse early embryo development was characterized by striking localization around the pronuclei in the equatorial section, in addition to larger areas of fluorescence deeper within the cytoplasm. In contrast, in diabetic mice, the ER displayed a significantly higher percentage of homogeneous distribution patterns throughout the entire ooplasm during oocyte maturation and early embryo development. In addition, a higher frequency of large ER aggregations was detected in GV oocytes and two cell embryos from diabetic mice. Conclusions: These results suggest that the diabetic condition adversely affects the ER distribution pattern during mouse oocyte maturation and early embryo development. Keywords: Endoplasmic reticulum, Maternal diabetes, Oocyte maturation, Early embryo
Background Women with poorly controlled type I diabetes encounter a higher prevalence of reproductive problems, such as infertility, miscarriage and offspring with congenital malformations [1]. Earlier studies showed that the diabetic condition adversely affects the development of pre- and post-implantation embryos in rodents [2-5]. Furthermore, numerous reports have suggested that in vitro-cultured two-cell stage embryos that were recovered from diabetic mice still experience significant delay in their progression * Correspondence: [email protected] † Equal contributors 2 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China Full list of author information is available at the end of the article
to the blastocyst stage and about 50% of two-cell stage embryos isolated from sub-diabetic rats were unable to develop to the eight-cell stage, even in a non diabetic tract [4,6]. Other studies revealed that preovulatory oocytes from chemically induced diabetic mice experience delayed germinal vesicle (GV) breakdown and abnormal cellular metabolism [4,7-9]. Oocytes from diabetic mice displayed a higher frequency of spindle defects and chromosome misalignment in meiosis, resulting in increased aneuploidy rates in ovulated oocytes [10]. To date, however, the effects of maternal diabetes on cytoplasmic
Data Loading...
