Posttranscriptional regulation of maternal Pou5f1/Oct4 during mouse oogenesis and early embryogenesis
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ORIGINAL PAPER
Posttranscriptional regulation of maternal Pou5f1/Oct4 during mouse oogenesis and early embryogenesis Yuki Takada1 · Rajan Iyyappan2 · Andrej Susor2 · Tomoya Kotani1,3 Accepted: 31 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Protein syntheses at appropriate timings are important for promoting diverse biological processes and are controlled at the levels of transcription and translation. Pou5f1/Oct4 is a transcription factor that is essential for vertebrate embryonic development. However, the precise timings when the mRNA and protein of Pou5f1/Oct4 are expressed during oogenesis and early stages of embryogenesis remain unclear. We analyzed the expression patterns of mRNA and protein of Pou5f1/Oct4 in mouse oocytes and embryos by using a highly sensitive in situ hybridization method and a monoclonal antibody specific to Pou5f1/ Oct4, respectively. Pou5f1/Oct4 mRNA was detected in growing oocytes from the primary follicle stage to the fully grown GV stage during oogenesis. In contrast, Pou5f1/Oct4 protein was undetectable during oogenesis, oocyte maturation and the first cleavage stage but subsequently became detectable in the nuclei of early 2-cell-stage embryos. Pou5f1/Oct4 protein at this stage was synthesized from maternal mRNAs stored in oocytes. The amount of Pou5f1/Oct4 mRNA in the polysomal fraction was small in GV-stage oocytes but was significantly increased in fertilized eggs. Taken together, our results indicate that the synthesis of Pou5f1/Oct4 protein during oogenesis and early stages of embryogenesis is controlled at the level of translation and suggest that precise control of the amount of this protein by translational regulation is important for oocyte development and early embryonic development. Keywords Mouse · Oocyte · Embryo · Maternal transcript · Translational regulation
Introduction Synthesis of proteins at the right time and right place is fundamentally important for promoting various cellular and developmental processes. This temporal and spatial protein expression is controlled by transcriptional machineries in the nucleus and translational machineries in the cytoplasm. Recent studies have demonstrated that the localization and translational control of mRNAs after transcription are critical for the spatial and temporal control of protein syntheses in many types of cells in diverse organisms (Kloc and Etkin * Tomoya Kotani [email protected] 1
Biosystems Science Course, Graduate School of Life Science, Hokkaido University, Sapporo 060‑0810, Japan
2
Laboratory of Biochemistry and Molecular Biology of Germ Cells, Institute of Animal Physiology and Genetics, CAS, Rumburska 89, 277 21 Libechov, Czech Republic
3
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060‑0810, Japan
2005; Besse and Ephrussi 2008; Buxbaum et al. 2015; Susor and Kubelka 2017). Pou5f1/Oct4 (also known as Oct3 and Oct5) was identified as one of the POU family transcriptional factors expressed specifically in ger
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