MCM7 Expression Is Altered in Rat After Spinal Cord Injury
- PDF / 861,846 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 40 Downloads / 223 Views
MCM7 Expression Is Altered in Rat After Spinal Cord Injury Jiajia Chen & Zhiming Cui & Weidong Li & Aiguo Shen & Guanhua Xu & Guofeng Bao & Yuyu Sun & Lingling Wang & Jianbo Fan & Jinlong Zhang & Longfei Yang & Zhiming Cui
Received: 29 January 2013 / Accepted: 11 March 2013 / Published online: 26 March 2013 # Springer Science+Business Media New York 2013
Abstract Minichromosome maintenance protein 7 (MCM7), a member of the minichromosome maintenance protein family, is essential for eukaryotic DNA replication initiation and the early stage of the elongation process. MCM7 participates in the cell cycle control of genome duplication. While it is ubiquitously expressed in all tissues, the biological function of MCM7 in the central nervous system is still with limited acquaintance. In the present study, we performed a spinal cord injury (SCI) model in adult rats. Western blotting indicated a marked alteration of MCM7 after SCI. Immunohistochemistry analysis revealed a wide distribution of MCM7 in the spinal cord. Double immunofluorescence staining showed that MCM7 immunoreactivity was increased predominantly in neurons, astrocytes, and microglia after SCI. We also examined the expression profiles of active caspase-3, proliferating cell nuclear antigen (PCNA), and Ki67, whose changes were correlated with the expression profiles of MCM7. Moreover, colocalization of MCM7/active caspase-3 was detected in neuronal nuclei (NeuN), and colocalization of MCM7/PCNA was detected in NeuN, glial fibrillary acidic protein, and CD11b, respectively. Our results suggest that MCM7 might be implicated in the apoptosis of neuron and proliferation of astrocytes and microglia after SCI. Keywords Spinal cord injury . MCM7 . Neuronal apoptosis . Proliferation . Rat Jiajia Chen and Zhiming Cui contributed equally to this work. J. Chen : Z. Cui : W. Li : G. Xu : G. Bao : Y. Sun : L. Wang : J. Fan : J. Zhang : L. Yang : Z. Cui (*) Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People’s Republic of China e-mail: [email protected] A. Shen Department of Immunology, Medical College, Nantong University, 226001, Nantong, Jiangsu, People’s Republic of China
Abbreviations MCM7 Minichromosome maintenance protein 7 SCI Spinal cord injury CNS Central nervous system RB Retinoblastoma RT Room temperature BSA Bovine serum albumin PCNA Proliferating cell nuclear antigen NeuN Neuronal nuclei GFAP Glial fibrillary acidic protein GAPDH Glyceraldehyde-3-phosphate dehydrogenase
Introduction Spinal cord injury (SCI) has a severely impact on the quality and expectancy of life and financial burden, which contributes to a major cause of disability and death in the world with limited treatment options (Rodriguez-Zayas et al. 2010; Khalatbary and Zarrinjoei 2012). Traumatic SCI causes tissue loss and associated neurological dysfunction through both mechanical damage and secondary biochemical and physiological responses (Ambrozaitis et al. 2006). Secondary injury follows the mechan
Data Loading...
