The Regenerative Effect of Trans-spinal Magnetic Stimulation After Spinal Cord Injury: Mechanisms and Pathways Underlyin
- PDF / 4,998,175 Bytes
- 20 Pages / 595.276 x 790.866 pts Page_size
- 29 Downloads / 190 Views
ORIGINAL ARTICLE
The Regenerative Effect of Trans-spinal Magnetic Stimulation After Spinal Cord Injury: Mechanisms and Pathways Underlying the Effect C. Chalfouh 1,2 & C. Guillou 3,4 & J. Hardouin 3,4 & Q. Delarue 1,2 & X. Li 5 & C. Duclos 1,2 & D. Schapman 4,6 & J.-P. Marie 1,2 & P. Cosette 3,4 & N. Guérout 1,2
# The Author(s) 2020
Abstract Spinal cord injury (SCI) leads to a loss of sensitive and motor functions. Currently, there is no therapeutic intervention offering a complete recovery. Here, we report that repetitive trans-spinal magnetic stimulation (rTSMS) can be a noninvasive SCI treatment that enhances tissue repair and functional recovery. Several techniques including immunohistochemical, behavioral, cells cultures, and proteomics have been performed. Moreover, different lesion paradigms, such as acute and chronic phase following SCI in wild-type and transgenic animals at different ages (juvenile, adult, and aged), have been used. We demonstrate that rTSMS modulates the lesion scar by decreasing fibrosis and inflammation and increases proliferation of spinal cord stem cells. Our results demonstrate also that rTSMS decreases demyelination, which contributes to axonal regrowth, neuronal survival, and locomotor recovery after SCI. This research provides evidence that rTSMS induces therapeutic effects in a preclinical rodent model and suggests possible translation to clinical application in humans. Keywords Rehabilitation . spinal cord injury . glial scar . magnetic stimulation and neuroregeneration
Abbreviations BDA biotinylated dextran amine DAPI 4′,6-diamidino-2-phénylindole GFAP glial fibrillary acidic protein Iba1 ionized calcium-binding adapter molecule 1 MBP myelin basic protein
* C. Chalfouh [email protected] * N. Guérout [email protected] 1
Normandie Univ, UNIROUEN, EA3830 GRHV, 76000 Rouen, France
2
Institute for Research and Innovation in Biomedicine (IRIB), 76000 Rouen, France
3
PISSARO Proteomic Facility, Normandie Univ, UNIROUEN, 76821 Mont-Saint-Aignan, France
4
Institute for Research and Innovation in Biomedicine (IRIB), Mont-Saint-Aignan, France
5
Department of Neurobiology, Care sciences and Society, BioClinicum, Karolinska Institutet, 17164 Stockholm, Sweden
6
Normandie Univ, UNIROUEN, SFR IRIB, Plateau PRIMACEN, F-76821 Mont-Saint-Aignan, France
PDGFRβ rMS rTSMS SCI WT
platelet-derived growth factorβ repetitive magnetic stimulation repetitive trans-spinal magnetic stimulation spinal cord injury wild type
Background Spinal cord injury (SCI) is an incurable disease which leads to a permanent loss of motor, sensation, and sensory functions below the injury level [1]. To date, no cure can be offered to paraplegic or tetraplegic patients. Thus, for many decades, researchers have conducted a substantial number of studies in order to understand the mechanisms responsible for the lack of recovery following SCI. Thereby, it has been reported that SCI induces multiple cellular and molecular responses as inflammation, neuronal death, demyelination, and scar form
Data Loading...
