Measurement of viscoelastic properties of injured mouse brain after controlled cortical impact
- PDF / 2,393,570 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 30 Downloads / 184 Views
Biophysics Reports
METHOD
Measurement of viscoelastic properties of injured mouse brain after controlled cortical impact Yu Chen1, Suhao Qiu1, Cheng Wang2, Xiaowei Li3, Yaohui Tang2, Yuan Feng1& 1
2
3
Institute for Medical Imaging Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
Received: 1 April 2020 / Accepted: 24 June 2020
Abstract
Mechanical properties of brain tissue can provide vital information for understanding the mechanism of traumatic brain injury (TBI). As mouse models were commonly adopted for TBI studies, a method to produce injury to the brain and characterize the injured tissue is desired. In this paper, a complete workflow of TBI induction, sample preparation, and biomechanical characterization is presented for measurement of the injured brain tissue. A controlled cortical impact device was used to induce injury to the brain. By setting the angle, speed, and position of the impact, the level of brain injuries could be controlled. Viscoelastic properties of both injured and non-injured brain tissues were measured using a ramp-hold indentation test. Regions of interests (ROIs) were tested and compared to contralateral corresponding counterparts. Methods introduced in this paper could be easily extended to produce and test a variety of other injured soft biological tissues.
Keywords Controlled cortical impact, Traumatic brain injury, Viscoelastic properties, Mouse model
INTRODUCTION Traumatic brain injury (TBI) is one of the major causes of morbidity and mortality worldwide, with more than 50 million patients each year and causing 400 billion dollars of global economy loss annually (Maas et al. 2017). TBI is induced by a mechanical process. Many studies have shown that mechanical properties of the brain are closely related to its development and diseases (Goriely et al. 2015). Biomechanical testing of the brain tissue could provide key physical parameters for the modeling and prediction of TBI (Ganpule et al. 2017; Lu et al. 2019; Madhukar and Ostoja-Starzewski 2019; Zhao et al. 2017; Zhou et al. 2019). Comparisons of mechanical properties between the healthy and injured brain tissues can provide important clues to understand & Correspondence: [email protected] (Y. Feng)
Ó The Author(s) 2020
the mechanism of TBI (Feng et al. 2017a; Qiu et al. 2020). Therefore, understanding the biomechanical properties of the brain tissue is important to decipher the myths of TBI. Animal models are indispensable for TBI research since injury-level experiments are impractical for human subjects. Despite the fact that the animal models cannot completely represent human brain injury, animal models of TBI still offer the best alternative to examine the biomechanical, cellular, and molecular mechanism of TBI-associated neuropathological progre
Data Loading...
