Mechanical adaptation of synoviocytes A and B to immobilization and remobilization: a study in the rat knee flexion mode
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Mechanical adaptation of synoviocytes A and B to immobilization and remobilization: a study in the rat knee flexion model Haodong Zhou1,2 · Guy Trudel2,3 · Odette Laneuville1,2 Received: 21 February 2020 / Accepted: 29 July 2020 © Springer Nature B.V. 2020
Abstract The objective of this study was to quantify the in vivo response of synoviocytes type A and B in the posterior joint capsule to knee immobilization and remobilization. Also, to correlate the immunohistochemical data with selected mRNA expression in the posterior joint capsule. Forty-two adult male Sprague–Dawley rats had one knee joint immobilized in flexion for durations of 1–4 weeks. Fifteen were harvested after immobilization and 15 were remobilized for 4 weeks. They were analyzed immunohistochemically with CD68 and CD55 antibodies as markers for synoviocytes type A and type B, respectively. Controls were 15 age-matched rats. The remaining 12 rats had their posterior capsule harvested and synoviocyte-specific CD68, CD55, and uridine diphosphoglucose dehydrogenase (UDPGD) mRNA expression was measured. Controls were 12 sham-operated knees. Knee immobilization for 2 weeks significantly increased synoviocytes A:B staining ratio compared to controls (3.88 ± 1.39 vs. 1.83 ± 0.76; p
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