Mechanism of Bile Acid Reabsorption in the Biliopancreatic Limb After Duodenal-Jejunal Bypass in Rats
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ORIGINAL CONTRIBUTIONS
Mechanism of Bile Acid Reabsorption in the Biliopancreatic Limb After Duodenal-Jejunal Bypass in Rats Tomotaka Ueno 1 & Naoki Tanaka 1 & Hirofumi Imoto 1 & Masamitsu Maekawa 2 & Atsushi Kohyama 1 & Kazuhiro Watanabe 1 & Fuyuhiko Motoi 1 & Takashi Kamei 1 & Michiaki Unno 1 & Takeshi Naitoh 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background Bile acids (BAs) are important in the metabolic effects of bariatric surgery. Most BAs are reabsorbed in the ileum and recycled back to the liver. We have reported that this enterohepatic circulation was shortened by duodenal-jejunal bypass (DJB), and the biliopancreatic (BP)-limb plays an important role in reabsorption of BAs. However, the mechanism of BA reabsorption in BP-limb remains uncertain. We aimed to investigate the mechanisms of BA reabsorption after DJB, especially focusing on carrier-mediated transport of BAs and the impact of the presence or absence of lipids on BA reabsorption. Methods Otsuka-Long-Evans-Tokushima fatty rats or Sprague-Dawley rats were assigned to a control group and DJB group. BA levels in the divided small intestine were quantified with liquid chromatography-mass spectrometry. Labeled BA was injected and perfused with BA transporter inhibitors or mixture of lipids in the isolated BP-limb, and bile was sampled and analyzed. Results Conjugated BA levels in the BP-limb were significantly higher than that of the control group. BA absorption tended to decrease by the apical sodium-dependent BA transporter inhibitor and was significantly decreased by the organic aniontransporting peptide (OATP) inhibitor. BA absorption tended to increase in the absence of lipid solutions compared with that in the presence of lipid solutions. Conclusion We attributed the increased BA reabsorption in the BP-limb to lack of food in the BP-limb, which contains concentrated BAs and no lipids. OATP played an important role in BA reabsorption in the BP-limb. Therefore, BAs would be reabsorbed in different manners after DJB. Keywords Bile acids . Biliopancreatic limb . Duodenal-jejunal bypass . Bile acid transporter . Organic anion-transporting peptide
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11695-020-04506-3) contains supplementary material, which is available to authorized users. * Naoki Tanaka [email protected]
Fuyuhiko Motoi [email protected] Takashi Kamei [email protected]
Tomotaka Ueno [email protected]
Michiaki Unno [email protected]
Hirofumi Imoto [email protected]
Takeshi Naitoh [email protected]
Masamitsu Maekawa [email protected] Atsushi Kohyama [email protected]
1
Department of Surgery, Tohoku University Graduate School of Medicine, 1-1, Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan
Kazuhiro Watanabe [email protected]
2
Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1, Seiryo-machi, Aoba-ku, Send
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