Meiosis
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedure
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David T. Stuart Editor
Meiosis Second Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Meiosis Second Edition
Edited by
David T. Stuart Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
Editor David T. Stuart Department of Biochemistry University of Alberta Edmonton, AB, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6338-6 ISBN 978-1-4939-6340-9 (eBook) DOI 10.1007/978-1-4939-6340-9 Library of Congress Control Number: 2016942558 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Gametogenesis is a truly remarkable differentiation program that produces haploid gametes from somatic diploid progenitor cells. A central and highly conserved feature of gamete formation is the chromosome divisions that occur during meiosis. The reduction in ploidy that results from two rounds of meiotic chromosome division without an intervening S-phase is essential to allow for the chromosome doubling that occurs upon fertilization or gamete fusion. The relatively large chromosomes of some organisms like the evening primrose Oenothera Biennis and the grasshopper Chorthippus brunneus have made the study of meiosis accessible through cytology investigations for over a century. Genetic approaches to the study of meiosis have a similar long and productive history. The dynamic movements and reorganization of the chromosomes that are observable dur
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