Metabolomics integrated with transcriptomics: assessing the central metabolism of marine red yeast Sporobolomyces pararo
- PDF / 1,982,618 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 85 Downloads / 132 Views
ORIGINAL PAPER
Metabolomics integrated with transcriptomics: assessing the central metabolism of marine red yeast Sporobolomyces pararoseus under salinity stress Chunji Li1,2,3 · Die Zhao4 · Jianyu Yan1 · Ning Zhang5 · Bingxue Li1 Received: 24 December 2019 / Revised: 7 September 2020 / Accepted: 1 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Salinity stress is one of the most serious environmental issues in agricultural regions worldwide. Excess salinity inhibits root growth of various crops, and results in reductions of yield. It is of crucial to understand the molecular mechanisms mediating salinity stress responses for enhancing crops’ salt tolerance. Marine red yeast Sporobolomyces pararoseus should have evolved some unique salt-tolerant mechanism, because they long-term live in high-salt ecosystems. However, little research has conducted so far by considering S. pararoseus as model microorganisms to study salt-tolerant mechanisms. Here, we successfully integrated metabolomics with transcriptomic profiles of S. pararoseus in response to salinity stress. Screening of metabolite features with untargeted metabolic profiling, we characterized 4862 compounds from the LC–MS/MS-based datasets. The integrated results showed that amino acid metabolism, carbohydrate metabolism, and lipid metabolism is significantly enriched in response to salt stress. Co-expression network analysis showed that 28 genes and 8 metabolites play an important role in the response of S. pararoseus, which provides valuable clues for subsequent validation. Together, the results provide valuable information for assessing the central metabolism of mediating salt responses in S. pararoseus and offer inventories of target genes for salt tolerance improvement via genetic engineering. Keywords Salinity stress · Sporobolomyces pararoseus · Salt tolerant · Metabolomics · Transcriptomics
Introduction Soil salinity is one of the major abiotic stresses in agricultural crop productivity and distribution around the world (Alharby et al. 2018). Developing salt-tolerant crops is
Communicated by Erko stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02082-9) contains supplementary material, which is available to authorized users. * Ning Zhang [email protected] * Bingxue Li [email protected] 1
College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China
Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China
2
essential for preventing the yield loss in food production (Yamaguchi and Blumwald 2005). Marine red yeast is tolerant to high salt stress and has evolved to form species-specific genetic mechanisms for the regulation of salt response (Kutty and Philip 2008). It is promising for understanding the salinity tolerant mechanisms of marine red yeast, for which contribute to the traditional breeding a
Data Loading...
