Metagenomic analysis of the dust particles collected from the suction tube and the suction funnel of a dermatological la
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ORIGINAL ARTICLE
Metagenomic analysis of the dust particles collected from the suction tube and the suction funnel of a dermatological laser smoke evacuator system Ga-Eun Lee 1,2 & Jin Ju Kim 1 & Hei Sung Kim 3,4,5
&
Woo Jun Sul 1
Received: 24 June 2020 / Accepted: 15 October 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract In the last few decades, there has essentially been an explosion in the use of lasers in medicine, especially in the area of cosmetic dermatology. Potentially harmful substances are liberated when tissues are vaporized with laser. This creates numerous risks, including the spread of infectious disease. Smoke evacuators are devices that capture and filter laser plume, thereby maintaining a safe environment for the surgical team and patient. Our aim was to characterize the microbial community structure within the suction tube and funnel of the smoke evacuator system, identify their origin, and evaluate pathogenicity. Dust particles were collected from the instruments with a cotton swab. DNA was extracted from the swabs and the transport media, and sequencing was performed using the Illumina HiSeq Xplatform. Metagenomic analysis was conducted using the Empowering the Development of Genomics Expertise (EDGE) Bioinformatics pipeline and custom Python scripts. The most abundant bacterial species were Micrococcus luteus and Brevibacterium casei in the suction tube, and Dermacoccus sp. Ellin 185 and Janibacter hoylei in the suction funnel. A total of 15 medium- to high-quality metagenome-assembled genomes (MAGs) were constructed where we found 104 antibiotic-resistant genes (ARGs) and 741 virulence factors. Findings indicate that the suction tube and funnel are likely a reservoir of virulence factor genes and ARGs, which can possibly be passed on to other bacteria via horizontal gene transfer. We would like to emphasize the health risk these microorganisms pose and the need to reevaluate the current hygiene standards with regard to the smoke evacuator system. Keywords Metagenomic analysis . Dust particles . Suction tube . Suction funnel . Dermatology . Laser . Smoke evacuator system Ga-Eun Lee and Jin Ju Kim contributed equally to this work as co-first authors. Hei Sung Kim and Woo Jun Sul work as co-corresponding authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10103-020-03165-1) contains supplementary material, which is available to authorized users. * Hei Sung Kim [email protected] * Woo Jun Sul [email protected] Ga-Eun Lee [email protected]
2
Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA
3
Dr Philip Frost Department of Dermatology and Cutaneous Surgery, Miami Itch Center, Miller School of Medicine, University of Miami, Miami, FL, USA
4
Department of Dermatology, Incheon St. Mary’s Hospital, The Catholic University of Korea, Seoul 06591, South Korea
5
Department of Biomedicine & Health Sciences, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, S
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