Metagenomic profiling of bacterial diversity and community structure in termite mounds and surrounding soils
- PDF / 2,063,136 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 37 Downloads / 228 Views
ORIGINAL PAPER
Metagenomic profiling of bacterial diversity and community structure in termite mounds and surrounding soils Ben Jesuorsemwen Enagbonma1 · Caroline Fadeke Ajilogba1 · Olubukola Oluranti Babalola1 Received: 28 March 2020 / Revised: 6 June 2020 / Accepted: 21 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The study focuses on analysis of the compositional and diversity of bacteria in termite mound soils in comparison with the surrounding soils to verify the assertion that the high nutrient concentrations in termite mound soils influence a complex diversity of microorganisms. Here, whole DNA was extracted from soil samples collected from termite mounds and their surrounding soils which were 10 m apart and subsequently, sequenced using shotgun metagenomic approach. Our findings showed that both environments have several soil bacterial phyla in common. However, Proteobacteria and Actinobacteria significantly dominated the termite mound soils and the surrounding soils, respectively, with Tenericutes peculiar to only the termite mound soils. Furthermore, Bergeyella, Gloeothece, Thalassospira, and Glaciecola genera were exclusively identified in the termite mound soil samples. Diversity analysis showed that bacterial composition was different among the four sites (phyla level). This study also revealed a lot of unclassified groups of bacteria and this could point to the presence of potentially novel species. The differences observed in the bacterial structure and diversity from this study may be ascribed to variances in the physicochemical nature existing between the two environments. Mapping out schemes to culture these unclassified groups of bacteria discovered from this study would possibly set the platform for the discovery of novel bacteria for biotechnological applications. Keywords Amplicon sequencing · Bacterial composition · Illumina platforms · Termitarium · Whole genome
Introduction Securing the integrity of soil is very vital because biodiversity services, climate stability, water, energy, and food security rely on its functioning (McBratney et al. 2014). When it comes to defining soil quality and fertility, the structure and function of soil bacterial communities are often used as pointers, due to the fast response of bacteria to environmental alterations (Zhu et al. 2017). Soil bacteria contribute immensely in regulating soil nutrient cycling, soil fertility, Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01994-w) contains supplementary material, which is available to authorized users. * Olubukola Oluranti Babalola [email protected] 1
Food Security and Safety Niche, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, Mmabatho 2735, South Africa
and plant health. Despite the contributions of soil bacteria including those present in termite mound soils in improving ecosystems, roughly 1% of them have been re
Data Loading...
