High-Throughput Sequencing Reveals the Diversity and Community Structure in Rhizosphere Soils of Three Endangered Plants
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High‑Throughput Sequencing Reveals the Diversity and Community Structure in Rhizosphere Soils of Three Endangered Plants in Western Ordos, China Daolong Xu1 · Xiaowen Yu2 · Junbo Yang1 · Xupeng Zhao1 · Yuying Bao1 Received: 9 September 2019 / Accepted: 25 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract China’s western Ordos is a distribution area for the extremely precious remnants of ancient Asian environments, which in endangered plant species and complex ecosystems. Accordingly, in this study, we collect three endangered plants belonging to the Zygophyllaceae family, namely Tetraena mongolica, Sarcozygium xanthoxylon, and Nitraria tangutorum Bobr. High-throughput sequencing technology was applied to study microbial diversity in these plant rhizosphere soils. Analysis of species composition abundance, Alpha diversity, Beta diversity and microbial structure are analyzed. 2428 bacterial OTUs and 1256 fungal OTUs are obtained from the rhizosphere soils, and the bacterial and fungal sequencing coverage is above 99%. Bacilli are the most abundant (86.91%) in the bacterial community. The fungal community has significant differences in three plants. The abundances of the genus Dothideomycetes in the rhizosphere soils of Tetraena mongolica and Sarcozygium xanthoxylon plants are the highest, which are 44.57% and 37.69%, respectively. Thus, Dothideomycetes is the dominant bacteria in the community. The genus Sordariomycetes in the rhizosphere soil is the dominant fungi with a relative abundance of 41.04%. Redundancy analysis revealed that microbial communities were closely related to environmental factors. Overall, this study bring new insights into the relationship between rhizosphere soils microbial diversity and environment to improving the adaptability of the endangered plants in survival environment.
Introduction The endemic plant species are mostly distributed in the Western Ordos, an arid region of China. The area is located on the eastern edge of the Asian-African desert, a transitional area for the desertification of the Western Ordos and the grassland of the East Alashan [1, 2]. It is also home to the ancient Mediterranean relict plants, such as Tetraena mongolica Maxim, Helianthemum songaricum Schrenk, Potaninia mongolica Maxim, and Ammopiptanthus mongolicus (Maxim. ex Kom.) [3, 4]. Over a long period of evolution, plants have adapted to the extreme conditions. At present, the area is rich in coal resources, but the ecological
* Yuying Bao [email protected] 1
Sch Life Sci, Minist Educ, Key Lab Forage & Endem Crop Biotechnol, Inner Mongolia University, 235 West Univ Rd, Hohhot 010021, Inner Mongolia, People’s Republic of China
School of Ecology and Environment, Inner Mongolia University, Hohhot, People’s Republic of China
2
environment is relatively fragile [5, 6]. In recent years, with the increment of the human activity affected, the further development of mineral resources, the continuous expansion of industrial parks and continuous grazing, the habitat of the
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