Metagenomics analysis of fungal communities associated with postharvest diseases in pear fruits under the effect of mana
- PDF / 1,353,154 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 96 Downloads / 182 Views
ORIGINAL PAPER
Metagenomics analysis of fungal communities associated with postharvest diseases in pear fruits under the effect of management practices Antonios Zambounis1 · Ioannis Ganopoulos2 · Athanasios Tsaftaris3 · Dimitrios Valasiadis4 · Panagiotis Madesis4 Received: 10 November 2019 / Revised: 25 May 2020 / Accepted: 16 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract An amplicon metagenomic approach based on the ITS1 region of fungal rDNA was employed to identify the composition of fungal communities associated with diseases of pear fruits during postharvest storage. The sampled fruits were harvested at an orchard using routine management practices involving treatments with various chemical fungicides and were transferred to a storage packinghouse. Effective tags of reading sequences clustered into 53 OTUs whereas Ascomycota was the dominant phylum (83.4%) followed by Basidiomycota (15.8%). Our results revealed that four genera, Penicillium, Rhodotorula, Alternaria and Cladosporium were the most abundant representing 59–95% of the relative abundance per sample. The interruption of chemical treatments during the last month before harvest altered the structure of the fungal community of fruits among untreated and treated samples, mainly in cases of relative abundance of Penicillium and Rhodotorula genera. We hypothesize that various antagonistic interactions might occur on fruit surfaces among the detected fungal genera whose relative abundances were affected by fungicide treatments. Interestingly, some common pre- and postharvest pear fungal pathogens were either less present (such as Moniliana), or undetected (such as Aspergillus, Venturia and Septoria) in untreated and treated samples. Keywords Carposphere fungal communities · Metagenomics
Introduction Pear (Pyrus communis L.) is an important deciduous tree crop worldwide and its global production reached 24,168.309 tons in 2017 (FAOSTAT 2019). Pear fruits are susceptible to several fungal pathogens, both in the field and during postharvest storage, and thus the application of Communicated by Erko Stackebrandt. * Antonios Zambounis [email protected] 1
Institute of Plant Breeding and Genetic Resources, Department of Deciduous Fruit Trees, ELGO-DEMETER, 59035 Naoussa, Greece
2
Institute of Plant Breeding and Genetic Resources, ELGO-DEMETER, Thermi, 57001 Thessaloniki, Greece
3
American Farm School, Perrotis College, 57001 Thessaloniki, Greece
4
Institute of Applied Biosciences, CERTH, Thermi, 57001 Thessaloniki, Greece
fungicide treatments is a prerequisite to avoid significant economic losses (Llorente et al. 2006; Sardella et al. 2016; Nabi et al. 2017). More precisely, postharvest diseases are caused by either latent infections that occur in the orchard but remain quiescent during the growing season or infections from wounds during harvest and storage practices (Simson and Straus 2010; Sutton et al. 2014; Ewekeye et al. 2016). For example, Penicillium expansum is the main causal agent of blue mol
Data Loading...
