Metal center ion effects on photoinactivating rapidly growing mycobacteria using water-soluble tetra-cationic porphyrins
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Metal center ion effects on photoinactivating rapidly growing mycobacteria using water-soluble tetra-cationic porphyrins Kevim Bordignon Guterres . Grazielle Guidolin Rossi . Marli Mati ko Anraku de Campos . Kelly Schineider Moreira . Thiago Augusto Lima Burgo . Bernardo Almeida Iglesias
Received: 1 June 2020 / Accepted: 16 September 2020 / Published online: 26 September 2020 Ó Springer Nature B.V. 2020
Abstract Rapidly growing mycobacteria (RGM) are pathogens that belong to the mycobacteriaceae family and responsible for causing mycobacterioses, which are infections of opportunistic nature and with increasing incidence rates in the world population. This work evaluated the use of six water-soluble cationic porphyrins as photosensitizers for the antimicrobial photodynamic therapy (aPDT) of four RGM strains: Mycolicibacterium fortuitum, Mycolicibacterium smeagmatis, Mycobacteroides abscessus subs. Abscessus, and Mycobacteroides abscessus subsp. massiliense. Experiments were conducted with an adequate concentration of photosensitizer under white-light irradiation conditions over 90 min and the results showed that porphyrins 1 and 2 (M = 2H or ZnII ion) were the most effective and significantly reduced the concentration of viable mycobacteria. The
K. B. Guterres G. G. Rossi M. M. k. A. de Campos Laboratory of Mycobacteriology, Universidade Federal de Santa Maria, Av. Roraima 1000, Campus Camobi, Santa Maria, RS 97105-900, Brazil K. S. Moreira T. A. L. Burgo Coulomb Electrostatic and Mechanochemical Laboratory, Universidade Federal de Santa Maria, Av. Roraima 1000, Campus Camobi, Santa Maria, RS 97105-900, Brazil B. A. Iglesias (&) Laboratory of Bioinorganic and Porphyrinic Materials, Universidade Federal de Santa Maria, Av. Roraima 1000, Campus Camobi, Santa Maria, RS 97105-900, Brazil e-mail: [email protected]
present work shows the result is dependent on the metal-center ion coordinated in the cationic porphyrin core. Moreover, we showed by atomic force microscopy (AFM) the possible membrane photodamage caused by reactive oxygen species and analyzed the morphology and adhesive force properties. Tetrapositively charged and water-soluble metalloporphyrins may be promising antimycobacterial aPDT agents with potential applications in medical clinical cases and bioremediation. Keywords Tetra-cationic metalloporphyrins Mycobacterial photoinactivation Photodynamic therapy Abbreviations MIC ROS RGM PS aPDT AFM EFM LJ AA Man KI TTC M. fortuitum
Minimum inhibitory concentration Reactive oxygen species Rapidly growing mycobacteria Photosensitizers Antimicrobial photodynamic therapy Atomic force microscopy Electrostatic force microscopy Lo¨wenstein-Jensen Ascorbic acid Mannitol Potassium iodide 2,3,5-Triphenyltetrazolium chloride Mycolicibacterium fortuitum
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M. abscessus M. massiliense M. smeagmatis
Biometals (2020) 33:269–282
Mycobacteroides abscessus subs. abscessus Mycobacteroides abscessus subsp. massiliense Mycolicibacterium smeagmatis
Introducti
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