Micro-patterned hydroxyapatite/silk fibroin coatings on Mg-Zn-Y-Nd-Zr alloys for better corrosion resistance and cell be
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RESEARCH ARTICLE
Micro-patterned hydroxyapatite/silk fibroin coatings on Mg–Zn–Y–Nd–Zr alloys for better corrosion resistance and cell behavior guidance Lei CHANG (✉)1,2,3, Xiangrui LI1,2, Xuhui TANG1, He ZHANG1, Ding HE1, Yujun WANG1, Jiayin ZHAO1, Jingan LI1,2,3, Jun WANG1,2,3, Shijie ZHU1,2,3, Liguo WANG1,2,3, and Shaokang GUAN (✉)1,2,3 1 School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China 2 Henan Key Laboratory of Advanced Magnesium Alloy, Zhengzhou 450001, China 3 Key Laboratory of Materials Processing and Mold Technology (Ministry of Education), Zhengzhou University, Zhengzhou 450001, China
© Higher Education Press 2020
ABSTRACT: In this study, a micro-patterned hydroxyapatite/silk fibroin (HA-SF) coating was firstly fabricated on the surface of Mg-Zn-Y-Nd-Zr alloy by template-assisted electrospraying technique coupling with spin coating technique. Two types of micropatterns were achieved with high contour accuracy, namely HA-SF(line-pattern) and HASF(dot-pattern). The microstructure, composition, surface wettability and corrosion behaviors of the coatings were investigated by SEM, EDS, FTIR, XRD, water contact angle and potentiodynamic polarization test. The results revealed the hydrophilic nature of coatings and two orders of magnitude reduction of corrosion density (icorr) as compared with that of the substrate. All the micro-patterned surfaces promoted the attachment of MC3T3-E1 cells with visible filopodia after 1 d incubation. In addition, coatings with line pattern exhibited the superior guidance to cell migration as compared to dot pattern, and the preference of cell attachment in the convex zone was observed. In summary, the obtained micro-patterned HA-SF coatings possessed the remarkably improvement of anticorrosion ability and good efficacy in guidance of cell attachment and alignment, which can serve as a promising strategy for cellular response modulation at the interface of magnesium-based implants and bone. KEYWORDS: magnesium alloy; silk fibroin; hydroxyapatite; micro-patterned surface; electrospraying technique
Contents 1 Introduction 2 Materials and methods 2.1 Materials and chemicals
Received June 24, 2020; accepted August 7, 2020 E-mails: [email protected] (L.C.), [email protected] (S.G.)
2.2
Preparation of micro-patterned HA-SF coatings on Mg–Zn–Y–Nd–Zr alloy 2.3 Coating characterization 2.4 Corrosion characterization 2.5 In vitro assessment of cell-surface interaction 2.6 Statistical analysis 3 Results and discussion 3.1 Surface analysis 3.2 Electrochemical behavior
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Front. Mater. Sci.
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Guidance effect of cell attachment and alignment on micro-patterned HA-SF coatings 4 Conclusions Acknowledgements References
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Introduction
Magnesium (Mg) and its alloys have been explored as the potential clinical implants for nearly two centuries due to the good biocompatibility and bioactivity [1], especially in the orthopedic applications in virtue of the mechanical similarity to natural cortical bone [2]. In addition, it can degrade in physiologic
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