Microanatomy and Function of the Spleen
This publication provides the first comprehensive review of human splenic microanatomy in correlation with up-to-date information on the immunological function of different splenic compartments. The authors provide an inter-species comparison of splenic m
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Vol. 151
Editors F. Beck, Melbourne D. Brown, Charlestown B. Christ, Freiburg W. Kriz, Heidelberg E. Marani, Leiden R. Putz, Munchen Y. Sano, Kyoto T. H. Schiebler, Wurzburg K. Zilles, Dusseldorf
Springer-Verlag Berlin Heidelberg GmbH
Birte Steiniger Peter Barth
Microanatomy and Function of the Spleen With 10 Figures and 2 Tables
i
Springer
BIRTE STEINIGER
Institut fur Anatomie und Zellbiologie Robert-Koch-StraBe 6 D-3S033 Marburg, Germany (e-mail: [email protected] ) PETER BARTH
Medizinisches Zentrum fur Pathologie BaldingerstraBe D-3S043 Marburg, Germany
Library of Congress-Cataloging-in-Publication-Data Steiniger, Birte, 1955- . Microanatomy and function of the spleen / Birte Steiniger, Peter Barth. p. cm. - (Advances in anatomy, embryology, and cell biology; v. 151) Includes bibliographical references and index. ISBN 978-3-540-66161-0 ISBN 978-3-642-57088-9 (eBook) DOI 10.1007/978-3-642-57088-9 M.D. II. Title. III. Series. [DNLM: 1. Spleen-anatomy & histology. 2. Spleenphysiology. Wl AD433K v. 151 2000/ WH 600 S822m 1999] QM576.S84 1999 612.4'I-dc21 DNLMIDLC for Library of Congress This work is subject to copyright. Ali rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permis sion for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. © Springer-Verlag Berlin Heidelberg 2000
The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publishers cannot guarantee the accuracy of any information about dosage and application contained in this book. In every individual case the user must check such information by consulting the relevant literature. Production: PRO EDIT GmbH, D-69126 Heidelberg 27/3136-5432 1 O - Printed on acid-free paper SPIN: 10698805
Contents
1
Introduction ...................................
2
Materials and Methods .......................... 3
2.1 2.2 2.3 2.4 2.5 2.6
Animal Spleens ................................. 3 Human Spleens ................................. 3 Antibodies ..................................... 3 Single Staining Procedure for Immunohistology .... 6 Double Staining Procedure for Immunohistology ... 7 Demonstration ·of Acid Phosphatase in Cryostat Sections ............................. 8 Demonstration of Alkaline Phosphatase in Cryostat Sections ............................. 8
2.7
1
3
Microanatomical Compartments of the Rat Spleen..
9
3.1 3.1.1 3.1.2 3.1.3 3.2 3.3
White Pulp. .
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