Microfluidic Paper-Based Analytical Device for Histidine Determination
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Microfluidic Paper-Based Analytical Device for Histidine Determination Akimitsu Kugimiya 1 & Akane Fujikawa 1 & Xiao Jiang 2 & Z. Hugh Fan 2 & Toshikazu Nishida 3 & Jiro Kohda 1 & Yasuhisa Nakano 1 & Yu Takano 1 Received: 13 April 2020 / Accepted: 22 June 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective. Keywords Amino acid . Aminoacyl-tRNA synthetase . Biosensing . Laminated paper-based analytical device . Microfluidics
Introduction Paper-based microfluidic devices (PADs) have drawn intense research attention as analytical platforms [1–4]. PADs are of significant interest for several key reasons, including their simple and easy design and fabrication (e.g., the shape and length of the microfluidic path), as well as their lack of an external power source (because analysis is driven by capillary action between the water and paper). The cost of fabrication is also very low.
* Akimitsu Kugimiya kugimiya@hiroshima–cu.ac.jp
1
Department of Biomedical Information Sciences, Graduate School of Information Sciences, Hiroshima City University, 3-4-1 Ozuka-higashi, Asaminami-ku, Hiroshima 731-3194, Japan
2
Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, FL 32611-6250, USA
3
Department of Electrical and Computer Engineering, University of Florida, 219 Larsen Hall, Gainesville, FL 32611-6200, USA
Applied Biochemistry and Biotechnology
The analysis of the concentrations of free amino acids in biological samples is useful in clinical diagnostics [5, 6]. Histidine, for example, has important effects in the human body: the excess intake of histidine is the underlying mechanism of allergic response [7], and the pathogenesis of histidinemia is related to the histidine concentration in plasma [8]. Therefore, the analysis of amino acid concentrations in biological samples has diagnostic relevance. Highperformance liquid chromatography (HPLC) is generally used to analyze amino acids in biological fluids. However, currently available methods are time-consuming and could potentially delay diagnosis. Thus, the development of a simple and rapid analytical tool for measuring amino acid concentrations would be desirable for clinical diagnostics. We have reported an amino acid-specific enzymatic detection system based on aminoacyl-tRNA synthetase (aaRS) as the molecular recognition element [9–13]. aaRS are involved in the biosynthes
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