MicroRNA-151 Attenuates Apoptosis of Endothelial Cells Induced by Oxidized Low-density Lipoprotein by Targeting Interleu
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ORIGINAL ARTICLE
MicroRNA-151 Attenuates Apoptosis of Endothelial Cells Induced by Oxidized Low-density Lipoprotein by Targeting Interleukin-17A (IL-17A) Fanfeng Chen 1 & Xiaoning Ye 2 & Haote Jiang 2 & Guanxia Zhu 3 & Shouliang Miao 4 Received: 31 March 2020 / Accepted: 27 July 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Apoptosis of endothelial cells plays an important role in atherosclerosis (AS). MicroRNAs (miRNAs) have been confirmed to participate in the process of endothelial cell apoptosis. The main purpose of this study was to investigate the mechanism of miR151 and interleukin-17A (IL-17A) in apoptosis of atherosclerotic endothelial cells. The expression levels of miR-151 in human aortic endothelial cells (HAEC) after Ox-LDL treatment were detected by qRT-PCR. The expression levels of IL-17A were detected by qRT-PCR and Western blot. The effects of miR-151 and IL-17A on the apoptosis rate were detected by flow cytometry. The relationship between miR-151 and IL-17A was assessed by bioinformatics analysis and luciferase assay. The expression levels of miR-151 in HAEC after Ox-LDL treatment were reduced, and the expression of IL-17A was upregulated. MiR-151 and si-IL-17A inhibited the apoptosis rate of aortic endothelial cells treated by Ox-LDL. MiR-151 and si-IL-17A reduced the expression levels of c-caspase-9, c-caspase-3, and BAX proteins in Ox-LDL-treated HAEC and increased the expression levels of Bcl-2. MiR-151 inhibited the apoptosis of endothelial cells in AS, and IL-17A was a new target for miR151. Our findings provided a potential treatment for atherosclerosis in the treatment of AS. Keywords miR-151 . IL-17A . Atherosclerosis . Endothelial cells . Apoptosis
Introduction Atherosclerosis (AS) is a disease that can cause serious harm to human health [1]. The most serious complications of AS are
Associate Editor Junjie Xiao oversaw the review of this article * Shouliang Miao [email protected] 1
Department of Vascular Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou City 325015, Zhejiang Province, People’s Republic of China
2
The First Clinical Medical Institute, Wenzhou Medical University, Wenzhou City 325015, Zhejiang Province, People’s Republic of China
3
Department of Oncology, The First Clinical Medical College of Wenzhou Medical University, Wenzhou City 325015, Zhejiang Province, People’s Republic of China
4
Department of Radiology, The First Affiliated Hospital, Wenzhou Medical University, Nanbaixiang Street, Ouhai District, Wenzhou City 325015, Zhejiang Province, People’s Republic of China
myocardial infarction and stroke caused by acute arterial occlusion [2]. With improved standard of living and the changes of eating habits, the incidence of atherosclerosis and related diseases is also increasing year by year [3]. It not only seriously endangers people’s life and health but also brings huge economic burden to the society [4]. Therefore, it is urgent to fully understand the pathogenesis of AS. Endothelium cells provide
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