Mid-Infrared Imaging Is Able to Characterize and Separate Cancer Cell Lines
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ORIGINAL ARTICLE
Mid-Infrared Imaging Is Able to Characterize and Separate Cancer Cell Lines E. Kontsek 1
&
A. Pesti 1 & M. Björnstedt 2 & T. Üveges 3 & E. Szabó 3 & T. Garay 1 & P. Gordon 4 & S. Gergely 3 & A. Kiss 1
Received: 23 January 2020 / Accepted: 19 May 2020 # The Author(s) 2020
Abstract Malignancies are still responsible for a large share of lethalities. Macroscopical evaluation of the surgical resection margins is uncertain. Big data based imaging approaches have emerged in the recent decade (mass spectrometry, two-photon microscopy, infrared and Raman spectroscopy). Indocianine green labelled MS is the most common approach, however, label free midinfrared imaging is more promising for future practical application. We aimed to identify and separate different transformed (A-375, HT-29) and non-transformed (CCD986SK) cell lines by a label-free infrared spectroscopy method. Our approach applied a novel set-up for label-free mid-infrared range classification method. Transflection spectroscopy was used on aluminium coated glass slides. Both whole range spectra (4000–648 cm−1) and hypersensitive fingerprint regions (1800–648 cm−1) were tested on the imaged areas of cell lines fixed in ethanol. Non-cell spectra were possible to be excluded based on mean transmission values being above 90%. Feasibility of a mean transmission based spectra filtering method with principal component analysis and linear discriminant analysis was shown to separate cell lines representing different tissue types. Fingerprint region resulted the best separation of cell lines spectra with accuracy of 99.84% at 70–75 mean transmittance range. Our approach in vitro was able to separate unique cell lines representing different tissues of origin. Proper data handling and spectra processing are key steps to achieve the adaptation of this dye-free technique for intraoperative surgery. Further studies are urgently needed to test this novel, marker-free approach. Keywords Cancer . FT-IR . Fingerprint region . Transflectance
Introduction Malignancies represent a huge burden on the society and the costs of novel oncological therapies are ever increasing [1]. Moreover, precise, personalized and fast multidisciplinary team decision is required to start the proper therapy [2].
* E. Kontsek [email protected] 1
2nd Department of Pathology, Semmelweis University, Budapest, Hungary
2
Laboratory for Clinical Pathology and Cytology, Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
3
Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Budapest, Hungary
4
Department of Electronics Technology, Budapest University of Technology and Economics, Budapest, Hungary
Positive surgical margin (PSM) means that the resection margin is not tumor-free. This information is delivered by the pathology report and influences the therapeutical decision [3]. In the operating rooms the routine pathology background i
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