Human Cell Culture Cancer Cell Lines Part 3: Leukemias and Lymphomas
This book describes all human leukemia-lymphoma cell lines that have been established and that grow continuously under standardised in vitro conditions. These lines are derived from cells belonging to all the major hematopoietic cell lineages, i.e. B- and
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Human Cell Culture Volume 3
The titles published in this series are listed at the end of this volume.
Human Cell Culture Volume III Cancer Cell Lines Part 3: Leukemias and Lymphomas edited by
John R.W. Masters University College London, UK
and
Bernhard O. Palsson Department of Bioengineering, University of California San Diego, USA
KLUWER ACADEMIC PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW
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0-306-46877-8 0-792-36225-X
.OXZHU$FDGHPLF3XEOLVKHUV 1HZ 80 in NCI-H929, 50 in LP1, 40–50 in MT3, 40 in OH-2,23 in LB-831 and LB-832, about 20 in 15 ( -chains) in RPMI 8226, 5–10 in EJM, 4–9 ( chains) in MM. 1, 4–9 in U-266,4 in UTMC-2, about 3 in KPMM2, 1.5 in U-1958, about 1 in SK-MM-2 cell line and < 1
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Multiple Myeloma Cell Lines
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Multiple Myeloma Cell Lines
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in UCD-HL461. Some MM cell lines are Ig non-producers (n = 9), reflecting the status (nonsecretory) in the MM patient in four cases. In other cases, the Ig production was lost during establishment (Table 2). Transplantability in nude mice was reported in a few cases (DOBIL-6, KMS-5). Interestingly, heterotransplantability was reported in the subline S6B45 of MM-S1 with ectopic IL-6 cDNA and autonomous growth [48]. A number of the cell lines can be cloned and form colonies in soft agar or methyl cellulose (KMM-1, KMS-5, KMS-12, LB-831, LB-832, MM-S1, RPMI 8226) while MM-A1, MM- Yl, U-1957, U-1958 and U-1996 were only clonable in the presence of IL-6 or feeder cells. Phenotypic alterations (including improved growth rate, development of feeder cell independence, capacity for growth as colonies in soft agar, growth as subcutaneous tumors in nude mice) have also been reported to occur as a consequence of long term cultivation, as exemplified by the U-266(–1984) cell line [93–95]. In one of the cell lines, LP-1, PWM or TPA can induce differentiation. The expression of unique genes (amylase mRNA) associated with the occurrence
of hyperamylasemia in the patient, was found in three cell lines (AD3, a subclone of FR4), FR4, and KHM-1B (Table 6).
7. PUTATIVE BUT UNCONFIRMED CELL LINES As described, MM cell lines are phenotypically very heterogenous. However, they can be distinguished from LCLs (see Table 1). The following criteria should be used to distinguish MM from LCLs: (1) morphology; MM cells have a plasmablast/plasma cell morphology including eccentric nuclei with prominent nucleoli, a prominent RER and Golgi and perinuclear zone, (2) capacity for a high rate of Ig secretion, (3) surface antigen profile, (4) aneuploidy, with numerous structural and numerical aberrations and, most importantly (5) the lack of EBV. In some reports characterization by these criteria is insufficient. Taking these markers into account, this review distinguishes four categories of cell lines; (1) authentic MM cell lines, (2) EBV-positive lymphoblastoi
