Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium l
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tochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging 1
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Yulin Zhang , Jianyong Wang , Shijia Xing , Liuju Li , Shiqun Zhao , Wenzhen Zhu , 3 1,4 1,2* Kuo Liang , Yanmei Liu & Liangyi Chen 1
State Key Laboratory of Membrane Biology, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular
Medicine, Peking University, Beijing 100871, China; PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China; 3 Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China; Institute for Brain Research and Rehabilitation (IBRR), Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, Guangzhou 200062, China 2
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Received December 25, 2019; accepted February 27, 2020; published online April 8, 2020
Despite the wide application of super-resolution (SR) microscopy in biological studies of cells, the technology is rarely used to monitor functional changes in live cells. By combining fast spinning disc-confocal structured illumination microscopy (SD-SIM) 2+ 2+ with loading of cytosolic fluorescent Ca indicators, we have developed an SR method for visualization of regional Ca dynamics and related cellular organelle morphology and dynamics, termed SR calcium lantern imaging. In COS-7 cells 2+ stimulated with ATP, we have identified various calcium macrodomains characterized by different types of Ca release from endoplasmic reticulum (ER) stores. Finally, we demonstrated various roles of mitochondria in mediating calcium signals from 2+ different sources; while mitochondria can globally potentiate the Ca entry associated with store release, mitochondria also 2+ locally control Ca release from the neighboring ER stores and assist in their refilling processes. 2+
super-resolution imaging, ER Ca release, mitochondrial, sequential response Citation:
Zhang, Y., Wang, J., Xing, S., Li, L., Zhao, S., Zhu, W., Liang, K., Liu, Y., and Chen, L. (2020). Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging. Sci China Life Sci 63, https://doi.org/10.1007/s11427-019-16594
INTRODUCTION Recently, emerging super-resolution (SR) imaging techniques have been used to explore intricate subcellular structures, such as the organization of the actin–spectrin-based membrane skeleton in the dendrites and soma of neurons (Han et al., 2017). However, any increase in spatial resolution requires a higher number of photons, thus limiting the majority of SR microscopy methods to structural imaging.
*Corresponding author (email: [email protected])
Because there are limits to the maximal emission photon fluxes of all fluorophores, current SR microscopy may not capture sufficient photons to monitor dynamic changes in 2+ cellular second messages such as Ca and cAMP in a timely manner. 2+ The “Ca microdomain” was detected for the first time indirectly in squid presynaptic termina
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