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Michal Minczuk Joanna Rorbach Editors

Mitochondrial Gene Expression Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK

For further volumes: http://www.springer.com/series/7651

For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.

Mitochondrial Gene Expression Methods and Protocols

Edited by

Michal Minczuk Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK

Joanna Rorbach Division of Molecular Metabolism, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna, Sweden; Max Planck Institute Biology of Ageing - Karolinska Institutet Laboratory, Karolinska Institutet, Stockholm, Sweden

Editors Michal Minczuk Medical Research Council Mitochondrial Biology Unit University of Cambridge Cambridge, UK

Joanna Rorbach Division of Molecular Metabolism Department of Medical Biochemistry and Biophysics Karolinska Institutet Solna, Sweden Max Planck Institute Biology of Ageing - Karolinska Institutet Laboratory Karolinska Institutet Stockholm, Sweden

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-0833-3 ISBN 978-1-0716-0834-0 (eBook) https://doi.org/10.1007/978-1-0716-0834-0 © Springer Science+Business Media, LLC, part of Springer Nature 2021 The chapter 7 is licensed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/). For further details see license information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in t