Human Cell Culture Protocols
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of H
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MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Human Cell Culture Protocols Third Edition Edited by
Ragai R. Mitry Institute of Liver Studies, King’s College Hospital, London, UK
Robin D. Hughes Institute of Liver Studies, King’s College London School of Medicine, London, UK
Editors Ragai R. Mitry, Ph.D Institute of Liver Studies King’s College Hospital London, UK [email protected]
Robin D. Hughes, Ph.D Institute of Liver Studies King’s College London School of Medicine London, UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-366-0 e-ISBN 978-1-61779-367-7 DOI 10.1007/978-1-61779-367-7 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011939785 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Dedication My sincere thanks to my mother Elizabeth, sister Antoinette, and brother Nabil for their love, encouragement, and support throughout my studies and career. Ragai R. Mitry
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Preface Welcome to the 3rd edition of Human Cell Culture Protocols. The 1st edition was published in 1996 with Gareth Jones as editor and the 2nd edition in 2004 with Joanna Picot as editor. We hope our edition is a worthy addition to this series. Some might say, “Why do we need another book on human cell culture?” Human cell culture is not a new topic, but development of new molecular techniques and reagents which can be used to investigate cell function and the intracellular mechanisms responsible make it a continuing requirement. Human stem cells have become an important area for cell culture but are not described here as they are dealt with by other volumes. We describe protocols for isolation and culture of a range of primary cells from human tissues. It is impossible to include all cell types, and, for instance, we have not included neural cells or cancer cells. Of increasing importance, the techniques can be adapted to obtain cell cultures from diseased tissues to help understand the pathogenetic mechanisms involved. A number of the chapters are new versions of
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