Modeling of the effect of bio-conjugation to anti-interleukin-10 antibodieson the photoluminescence of CdSe/ZnS quantum
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Modeling of the effect of bio-conjugation to anti-interleukin-10 antibodies on the photoluminescence of CdSe/ZnS quantum dots Tetyana V. Torchynska1, Yuri V. Vorobiev2, and Paul P. Horley3 1
ESFM Instituto Politécnico Nacional, México, D.F. 07738, México
2
CINVESTAV Instituto Politécnico Nacional, Querétaro, QRO 76230, México
3
CIMAV Chihuahua / Monterrey, Chihuahua, CHIH 31109, México
ABSTRACT Bio-conjugated CdSe/ZnS core/shell quantum dots (QDs) attract essential scientific interest due to their possible nano-medicine applications, including selective highlighting of affected tissues and targeted drug delivery to the certain type of cells. The paper is focused on the theoretical description of the blue shift observed in the luminescence spectra of CdSe/ZnS QDs upon their bio-conjugation with the anti-interleukin-10 antibodies. We propose a model that describes the ground state of the exciton confined in a quantum dot and explaining the bioconjugation phenomenon by the change of the effective confinement volume. INTRODUCTION The modern developments in nano-technology allow the numerous promising applications in the field of biology and medicine, which in the future may bring an unprecedented development of nano-medicine [1, 2]. This novel technique will allow targeted drug delivery to cells with certain parameters, as well as improvements in diagnostics and localization of affected tissues in the human body. To achieve these aims, it is necessary to make a bridge between the immunity-related agents (such as cytokines) and nano-particles with predefined properties. The key issue for nano-particles intended for biological use is to ensure their stability, which naturally suggests formation of core-shell structures with a certain enveloping layer required to isolate a nano-particle. The core defines the desired properties of the system – it may be the material with outstanding luminescence or magnetic properties for precise detection of particle’s location inside the body; on the other hand, the core can be used as a reservoir for the medicine used in targeted drug delivery applications. In this paper, we focus on the antibody molecules to the interleukin 10 (IL-10). Interleukin-10 molecules (related to cytokines) participate in the inter-cellular communication. Cytokines are being produced at the surface of one cell and interact with the receptors of its neighbors, triggering an inter-cell response. The cytokines of interleukin family (Fig. 1) participate in the immuno-regulation and control inflammatory processes [3]. The studies earlier realized proved the important role of IL-10 in blocking inflammatory and allergic reactions [4]. We used the antiIL-10 antibodies (antihuman IL-10, Rt IgG1, clone JES3-9D7, code RHCIL1000) to perform the bio-conjugation with CdSe/ZnS core/shell QDs using the commercially available conjugation kit [5] with QDs characterized by 655 nm emission.
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Figure 1. Protein Database (PDB) rendering of Interleukin-10 (image courtesy Wikipedia)
Figure 2. Comparison of photoluminescenc
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