Molecular breeding of a high oleic acid soybean line by integrating natural variations
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Molecular breeding of a high oleic acid soybean line by integrating natural variations Haiyang Nan & Sijia Lu & Chao Fang & Zhihong Hou & Cen Yang & Qian Zhang & Baohui Liu & Fanjiang Kong
Received: 27 June 2020 / Accepted: 26 August 2020 # Springer Nature B.V. 2020
Abstract Soybean is an important crop used for oil production. Alterations of the fatty acids, especially increased oleic acid content, can improve the nutritional quality and oxidative stability of soybean oil. During seed development, two genes, encoding FAD2-1A and FAD2-1B, are mainly responsible for the transformation from oleic acid to linoleic acid, and the combination of fad2-1a and fad2-1b is the key factor for increasing oleic acid content. To breed high oleic acid varieties, we analyzed the haplotype of FAD2-1A and FAD2-1B among 1250 soybean accessions and detected a novel mutation in the FAD2-1A gene that we described as fad2-1a/W254Stop. By focusing on the mutation fad2-
1a/W254Stop and the reported fad2-1b allele, we developed two molecular markers. Based on these markers, we selected one line (435) from a cross between the fad2-1a(W254Stop) allele and the existing fad2-1 allele. The oleic acid content of ‘435’ was 91.03%. The ‘435’ line was used as a donor parent, and an elite soybean cultivar ‘Heinong51’ was selected as the recurrent parent. After three backcrosses, we detected an individual with high oleic acid content (75%) and high yield. We named this individual ‘Fuhang3’. This study generated material for breeding high oleic acid soybean varieties and improved the rate of breeding novel soybean varieties.
Haiyang Nan and Sijia Lu contributed equally to this work.
Keywords Soybean . Oleic acid . FAD2-1A . FAD21B . Molecular marker
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01168-y) contains supplementary material, which is available to authorized users. H. Nan (*) : S. Lu : C. Fang : Z. Hou : C. Yang : B. Liu : F. Kong Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, China e-mail: [email protected] Q. Zhang Heilongjiang Full Harvest Agrosciences Co., Ltd, Harbin, China B. Liu : F. Kong (*) The Innovative Academy of Seed Design, Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China e-mail: [email protected]
Introduction Soybean (Glycine max (L.)) is an essential legume crop that provides human dietary proteins, animal feed, agricultural products, and vegetable oil (Amin et al., 2019). Soybean oil typically has a fatty acid profile consisting of 13% palmitic acid, 4% stearic acid, 20% oleic acid, 55% linoleic acid, and 8% linolenic acid (Fehr, 2007; Pham et al., 2010). Soybean seed oil from a typical representative sample includes five major fatty acids: 11% palmitic, 4% stearic, 25% oleic, 52% linoleic, and 8% linolenic (Fehr, 2007). The relatively high concentration of linoleic and α-linolenic acids (approximat
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