Overexpression of Maize ZmHDZIV14 Increases Abscisic Acid Sensitivity and Mediates Drought and Salt Stress in Arabidopsi
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ORIGINAL ARTICLE
Overexpression of Maize ZmHDZIV14 Increases Abscisic Acid Sensitivity and Mediates Drought and Salt Stress in Arabidopsis and Tobacco Peng Fang 1 & Huiping Yan 1 & Fenqi Chen 1 & Yunling Peng 1,2 Received: 16 April 2020 / Accepted: 28 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Homeodomain-leucine zipper (HD-Zip) IV transcription factors are integral in the plant response to abiotic stressors. In maize, few HD-Zip IV proteins have yet been reported, and the functions are not well understood. In the current study, we isolated ZmHDZIV14 from the HD-Zip IV transcription factor family from maize and characterized its role in the stress response. We conducted a phylogenetic analysis showing that there was high homology and thus likely similar functions between ZmHDZIV14 and AtHDG11. Heterologous overexpression of ZmHDZIV14 in Arabidopsis caused increased sensitivity to abscisic acid (ABA), tolerance of salt and mannitol at germination stage, and drought tolerance of seedlings. ZmHDZIV14 transgenic Arabidopsis lines placed under drought stress additionally displayed lower levels of malondialdehyde, higher proline, and relative water content, compared to the wild-type. Further, modified tobacco plants (35S::ZmHDZIV14) responded to drought conditions with appropriate stomatal changes. After drought was relieved, expression of P5CS1, RD22, RD29B, RAB18, NCED3, and ERD1 were upregulated in transgenic Arabidopsis. It can be seen ZmHDZIV14 expression resulted in physiological and molecular changes in the transgenic lines, which improved overall drought tolerance. In summary, our results indicate that ZmHDZIV14 is a transcriptional regulator that modifies an ABA-dependent signaling pathway to regulate the drought response in plants. Keywords Homeodomain-leucine zipper . ZmHDZIV14 . Maize . Drought tolerance . Salt tolerance . ABA
Introduction Peng Fang and Huiping Yan contributed equally to this work. Key Message • ZmHDZIV14 was isolated from maize HD-Zip IV transcription factor family for the first time. • Heterologous overexpression of ZmHDZIV14 in Arabidopsis increased tolerance of salt and mannitol at germination stage, and increased drought tolerance of seedlings. • ZmHDZIV14 modified ABA-dependent signaling pathway to regulate the drought response in plants. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11105-020-01252-9) contains supplementary material, which is available to authorized users. * Yunling Peng [email protected] 1
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China
2
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
Extreme abiotic stress conditions, including salinity and drought, can critically limit growth and productivity of plants. In response, plants have adapted to stress conditions by evolving complex responses in morphology, physiology, and gene expression (Ingram and Bartels 1996; Verslues et
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