Molecular characterization of tobacco ringspot virus from Iris ensata
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Molecular characterization of tobacco ringspot virus from Iris ensata Jingwen Yang & Dunhuang Fang & Jianguo Shen & Xihong Chen & Fangluan Gao
Accepted: 17 August 2020 # Koninklijke Nederlandse Planteziektenkundige Vereniging 2020
Abstract Tobacco ringspot virus (TRSV) is an important plant pathogen that causes severe diseases in tobacco plants. In recent years, this virus has been reported in a number of ornamental plants. However, it has never been reported from Japanese iris, a famous traditional ornamental plant distributed in Asian countries. In this study, the near-complete bipartite genome of a nepovirus (TRSV-Iris) infecting Japanese iris from Taiwan of China was determined. The RNA1 and RNA2 are 7512 and 3899 nucleotides in length, and encodes a polyprotein of 2303 and 1078 amino acids, respectively. Sequence analysis indicates that TRSV-Iris shares more than 80% amino acid sequence identity, the threshold values of the species demarcation for the genus Nepovirus, with known TRSV isolates either in the Jingwen Yang and Dunhuang Fang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10658-020-02105-7) contains supplementary material, which is available to authorized users. J. Yang : X. Chen : F. Gao (*) Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China e-mail: [email protected] D. Fang Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, China J. Shen (*) Technology Center of Fuzhou Customs District, Fuzhou 350001, China e-mail: [email protected]
conserved Pro-Pol or CP regions, respectively. Phylogenetic analysis indicates that TRSV-Iris is a TRSV isolate. Keywords Tobacco ringspot virus . Iris ensata . Nepoviruses . Phylogenetic analysis . GC and GDD motifs
The genus Nepovirus is classified into the subfamily Comovirinae of the family Secoviridae (Lefkowitz et al. 2018). The genome of nepovirures consists of two positive-strand RNAs with 5′-bound polypeptide (VPg) and 3′- poly-A tailed. Each of the two genomic RNA is translated into a single large polyprotein. Nepoviruses are traditionally grouped into three phylogenetic subgroups (namely subgroup A, subgroup B and subgroup C) based on the specific size of RNA 2, the presence/absence of middle and bottom (termed M and B, respectively) components in virions, and the specificity of cleavage sites (Sanfaçon et al. 2011). Tobacco ringspot virus (TRSV), the type member of the genus Nepovirus (Lefkowitz et al. 2018), has isometric particles with a diameter of about 28 nm. Its RNA1 encodes a replicase protein and RNA2 encodes a hypothetical peptide (2A), a movement protein (MP) and a coat protein (CP), respectively. TRSV can be transmitted in a variety of ways, including by sap inoculation, seed and the nematode vector (Li et al. 2014; McGuire et al. 1970). It has a wide host range and can infect at least 300 species including many economic crops. Viral diseases caused by this pathogen has posed a major threat
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