The complete S RNA and M RNA nucleotide sequences of a hippeastrum chlorotic ringspot virus (HCRV) isolate from Hymenoca
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BRIEF REPORT
The complete S RNA and M RNA nucleotide sequences of a hippeastrum chlorotic ringspot virus (HCRV) isolate from Hymenocallis littoralis (Jacq.) Salisb in China Ye Xu • Shi-gao Lou • Xiao-lin Li • Yuan-xian Zheng Wen-cheng Wang • Ya-ting Liu
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Received: 8 March 2013 / Accepted: 2 May 2013 Ó Springer-Verlag Wien 2013
Abstract An isolate of hippeastrum chlorotic ringspot virus (HCRV) named HLS1-2, causing necrotic spots on leaves of spider lily, was obtained and characterized in China. The complete S RNA and M RNAs of the HLS1-2 isolate are 2724 nt and 4741 nt in length, respectively. The HLS1-2 S RNA sequence is most closely related to that of HCRV, with 99 % identity. Viral proteins encoded by the M RNA are closely related to those of tomato yellow ring virus (TYRV), polygonum ringspot virus (PolRSV) and iris yellow spot virus (IYSV). Phylogenetic trees for the four viral proteins encoded by the S and M RNAs placed HCRV-HLS1-2 in a distinct cluster with IYSV, TYRV and PolRSV and provided further support for the subdivision of tospoviruses into American and Eurasian groups.
The nucleotide sequence data reported in this manuscript have been deposited at the NCBI/GeneBank under accession no. JX833564 and JX833565.
Electronic supplementary material The online version of this article (doi:10.1007/s00705-013-1756-x) contains supplementary material, which is available to authorized users. Y. Xu S. Lou W. Wang Y. Liu (&) College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China e-mail: [email protected] X. Li Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, China X. Li Yunnan Main Food Crop Seed Processing Technology Centre, Kunming 650205, China Y. Zheng China National Tobacco Corporation Yunnan Province Company, Lincang Branch, Lincang 677099, China
Tospovirus is the only genus of plant-infecting viruses in the family Bunyaviridae. Tospovirus particles are quasispherical, 80–120 nm in diameter, and enveloped by a lipid membrane [11, 28, 37]. They contain a tripartite genome of single-strand RNAs, termed S RNA, M RNA and L RNA according to their sizes. The S segment encodes two viral proteins in an ambisense gene arrangement: a non-structural protein (NSs) in the viral sense, which is a genesilencing suppressor [33], and the nucleocapsid (N) protein in the complementary sense [36]. The viral sense of the M segment encodes a second non-structure protein (NSm), which functions in the cell-to-cell movement of the virus [32], and the complementary sense encodes the glycoprotein precursor (Gn/Gc), which is located at the surface of the viral lipid membrane [2]. The negative-polarity L RNA encodes the RNA-dependent RNA polymerase (RdRp) [1]. Tospoviruses are spread worldwide, and the genus contains eight accepted species [3, 4, 11, 13, 22, 30, 35, 38] and 16 tentative species [3, 5–10, 12, 15, 17, 18, 20, 21, 23, 27, 31, 35, 40]. Tomato spotted wilt virus (TSWV), described in 1919, is the type species of the genus Tospovirus [4]
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