Molecular evidence of new freshwater turtle blood flukes (Digenea: Spirorchiidae) in the intermediate snail host Biompha
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HELMINTHOLOGY - ORIGINAL PAPER
Molecular evidence of new freshwater turtle blood flukes (Digenea: Spirorchiidae) in the intermediate snail host Biomphalaria occidentalis Paraense, 1981 in an urban aquatic ecosystem in Brazil Juliana Rosa Matias Ciccheto 1 & Bruno Henrique Mioto Stabile 2 & Fábio Fermino 3 & Thomaz Mansini Carrenho Fabrin 2 & Alessandra Valéria de Oliveira 2,4,5 & Ricardo Massato Takemoto 2,5,6 Rodrigo Junio da Graça 7
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Received: 13 May 2020 / Accepted: 25 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In this study, two potentially new species of turtle blood flukes (TBFs) (Digenea: Spirorchiidae) have been recorded from South Brazil. The spirorchiid parasites infect the vascular system of turtles, thereby compromising their health. The life cycle of these parasites is not well studied. The larval stage of cercaria is found in intermediate gastropod hosts, with some species presenting similar morphological characteristics, which can result in misinterpretations when using only morphological taxonomy for species identification. In this study, we recorded a single morphotype belonging to the family Spirorchiidae in Biomphalaria occidentalis in an urban aquatic ecosystem in Brazil. However, molecular data (28S rDNA and cytochrome oxidase subunit I) confirmed the presence of two species of Spirorchiidae in the sampled environment; both phylogenetically close to genera previously studied in freshwater turtles from the Peruvian Amazon. In this study, species characterization was possible because of molecular tools. We recommend using more than one molecular marker in future studies focusing on TBFs, which need attention about their evolutionary history and ecology to understand their distribution in South America. Keywords Freshwater gastropod . Neotropical TBF . Phylogeny . South America . Spirorchiidae
Section Editor: Christoph G. Grevelding Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s00436-02006945-4. * Bruno Henrique Mioto Stabile [email protected]
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Programa de Pós-graduação em Ecologia e Conservação, Universidade Federal do Paraná – UFPR, Curitiba Paraná Brazil
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Programa de Pós-graduação em Ecologia de Ambientes Aquáticos Continentais, Laboratório de Genética Molecular, Universidade Estadual de Maringá – UEM, Av. Colombo 5790, Bloco G90, Maringá Paraná CEP 87020-900 Brazil
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Secretaria de Estado da Educação e do Esporte do Paraná, Maringá Paraná Brazil
Thomaz Mansini Carrenho Fabrin [email protected]
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Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá – UEM, Maringá Paraná Brazil
Alessandra Valéria de Oliveira [email protected]
5
Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá – UEM, Maringá Paraná Brazil
Ricardo Massato Takemoto [email protected]
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Programa de Pós-graduação em Biologia Comparada, Universidade Estadual de Maringá – UEM, Maringá Paraná Brazil
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