Molecular identification and developmental expression patterns of growth hormone and its receptors in yellowtail kingfis
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Molecular identification and developmental expression patterns of growth hormone and its receptors in yellowtail kingfish (Seriola lalandi) Bin Wang1,2 · Yaxing Zhang1,3 · Quan Liu1,3 · Yongjiang Xu1,2 · Aijun Cui1,3 · Yan Jiang1,2 · Xuezhou Liu1,2 Received: 4 May 2020 / Accepted: 11 August 2020 © Springer Nature B.V. 2020
Abstract In fish and other vertebrates, growth hormone (GH) is an essential polypeptide required for normal growth and development. In an attempt to understand growth regulation in yellowtail kingfish (YTK), the full-length cDNA sequences encoding gh and its receptors (ghr1 and ghr2) were cloned, characterized and the expression profiles of these three genes were investigated during embryonic development. The full-length cDNA sequences of GH and its receptors were obtained by RT-PCR combined with RACE methord. YTK gh cDNA sequence was 852 base pairs (bp) that comprised an open reading frame (ORF) of 615 bp encoding a 204-amino acids (aa) precursor. The preprohormone compassed a signal peptide (17 aa) and the mature peptide (187 aa). YTK GHR1 protein consisted of a signal peptide (28 aa), an extracellular domain (222 aa), a single transmembrane domain (23 aa) and an intracellular domain (361 aa). GHR2 protein included 18 aa, 223 aa, 23 aa, and 321 aa, respectively. Tissue distribution analysis showed that the maximal level of gh expression was observed in the pituitary, and ghr1 mRNA was mainly detected in the liver, while ghr2 transcripts were most abundant in the gonad. Moreover, both ghr1 and ghr2 mRNAs were expressed in all embryonic stages and displayed different gene expression profiles. Overall, these results provide initial evidences for the involvement of the GH/GHR system in the early ontogeny of yellowtail kingfish. Keywords Growth hormone · Growth hormone receptor · Embryonic development · Seriola lalandi
Introduction Growth hormone (GH) plays an important role in various physiological processes, including growth, development, metabolism, reproduction, immunity and osmoregulation Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05729-4) contains supplementary material, which is available to authorized users. * Xuezhou Liu [email protected] 1
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 106 Nanjing Road, Qingdao 266071, China
2
Laboratory for Marine Fisheries and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
3
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
[1–3]. It is a single chain polypeptide and its biological actions are mediated by the membrane-bound GH receptors (GHRs). GHRs are single transmembrane proteins that consist of extracellular, transmembrane, and cytoplasmatic domains, and these characteristic structures were conserv
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