Molecular identification of infectious bronchitis virus isolated from respiratory diseases in some Iranian broiler flock
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ORIGINAL ARTICLE
Molecular identification of infectious bronchitis virus isolated from respiratory diseases in some Iranian broiler flocks Mohsen Ghorbiani1 · Zahra Boroomand1 · Mansour Mayahi1 · Masoud Reza Seyfi Abad Shapouri2 Received: 6 June 2020 / Accepted: 28 August 2020 © Springer Nature B.V. 2020
Abstract Infectious Bronchitis (IB) is an acute, highly contagious disease associated with respiratory signs in young chickens and reduced egg production and quality in layers. The purpose of this study was to isolate and identify the infectious bronchitis virus in broiler flocks with respiratory diseases in four provinces of Iran. The specimens from forty IB suspected flocks from different regions of Isfahan, East Azerbaijan, Golestan, and Khuzestan provinces were collected, and the trachea, lung, and cecal tonsils were sampled. The samples were inoculated into 9- to 11-day—old embryonated chicken eggs. After collecting the allantoic fluid, RT-PCR was carried out to detect IB viruses. The results showed that IBVs were isolated from 30% of the flocks in these four provinces. The positive samples, according to a partial S1 gene sequence, were more investigated. Comparing nucleotide and amino acid sequences showed that the four isolates had the most similarity to the Pakistani 793/B strain (GI-13 lineage). The three isolates had the most considerable similarity in amino acid and nucleotide sequences to Iraqi and Iranian QX-like viruses (GI-19 lineage). Two isolates had 96 to 98% resemblance to Iranian variant-2 (GI-23 lineage) isolates. One isolate was found to belong to the Massachusetts serotype (GI-1 lineage) having 100% similarity in its amino acid sequence to the Massachusetts serotypes in GenBank. The phylogenetic relationship of the isolates shows complexity and diversity concerning different sequences and geographical regions. Keywords Infectious bronchitis · Identification · Broiler flocks · Iran
Introduction Infectious bronchitis is considered one of the most common diseases of poultry. This disease often affects both vaccinated and unvaccinated flocks and caused significant economic losses in Iranian flocks in the last years [1]. The infectious bronchitis virus (IBV) belongs to group III of the genus Coronavirus of the family Coronaviridae, has a positive- sense, single-stranded RNA coding for four structural proteins: the spike glycoprotein (S), the membrane glycoprotein (M), the small envelope protein (E), and the nucleocapsid protein (N). The spike (S) Protein forms the main structure of the IBV proteins is located on the surface of the * Zahra Boroomand [email protected] 1
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2
viral envelope. This protein is the main factor in inducing the production of neutralizing antibodies, and is responsible for binding and entering the host cells. The S protein is broken down into
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