Molecular revolution in the diagnosis of microbial brain abscesses
- PDF / 599,092 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 201 Views
REVIEW
Molecular revolution in the diagnosis of microbial brain abscesses A. K. Mishra & H. Dufour & P.-H. Roche & M. Lonjon & D. Raoult & P.-E. Fournier
Received: 18 April 2014 / Accepted: 15 May 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract A brain abscess is a life-threatening infection, frequently with serious sequelae. Culture-based methods present many limitations and do not enable an exhaustive documentation of the bacterial flora. 16S rRNA-based amplification, cloning, and high-throughput sequencing have dramatically increased the number of identified agents of brain abscesses, showing that the causative flora is polymicrobial in up to 40 % of cases, with the presence of at least one anaerobic bacterium. In contrast, real-time polymerase chain reaction (PCR) technology is an appealing alternative to culture-based methods for diagnosing brain abscesses due to its speed, sensitivity, and specificity. Molecular typing is available for several bacterial and fungal genera, and this user-friendly tool is accessible for the clinical microbiology laboratory to diagnose microbes involved in a brain abscess. This article reviews the applications of the currently available tools for the etiological diagnosis of a brain abscess.
A. K. Mishra : D. Raoult : P.
Data Loading...
