Monocyte subsets study in children with Mycoplasma pneumoniae pneumonia
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ORIGINAL ARTICLE
Monocyte subsets study in children with Mycoplasma pneumoniae pneumonia Zhihua Wang 1 & Lei Yang 2 & Jing Ye 1,3 & Yushui Wang 1 & Yan Liu 1
# Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract The aim of this study was to evaluate the changes in the three subsets of monocyte (classical, intermediate, and non-classical) and the expression of human leukocyte antigen-DR (HLA-DR) on monocyte subsets during MP pneumonia in children. Monocyte subsets were analyzed in the peripheral blood of healthy volunteers and MP pneumonia patients at the stages of admission and remission after clinical therapy. They were defined as classical (CD14+CD16−), intermediate (CD14brightCD16+), and nonclassical (CD14dimCD16+) using flow cytometry. Furthermore, three subsets of monocyte were analyzed for the expression of HLA-DR. Patients with MP pneumonia at admission had a higher proportion of intermediate and non-classical monocytes than healthy subjects (all P < 0.05). The proportion of intermediate subset and non-classical subset was lower in MP pneumonia patients at remission than at admission (all P < 0.05). In comparison with the other monocyte subsets, intermediate subset showed a significantly higher percentage of HLA-DR in MP pneumonia patients at admission (P < 0.05). Further analysis revealed that the expression of HLA-DR on intermediate subset was lower in severe patients than in non-severe patients (P < 0.05).Our data has shown for the first time that MP pneumonia is associated with the increased proportion of non-classical and intermediate monocytes, indicating the involvement of monocyte-related mechanisms in the pathogenesis of this disease. Additionally, the decreased expression of HLA-DR on CD14brightCD16+ subset may be a potential indicator of the severity of MP pneumonia. Keywords Mycoplasma pneumoniae pneumonia . Monocyte subsets . Human leukocyte antigen-DR . Children
Introduction * Lei Yang [email protected] Zhihua Wang [email protected] Jing Ye [email protected] Yushui Wang [email protected] Yan Liu [email protected] 1
Tianjin Hospital of Integrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin, China
2
Institute of Acute Abdominal Diseases, Tianjin Integrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Nankai Sanwei Road, Tianjin 300100, China
3
Department of Pediatrics, The Second Hospital Affiliated to Tianjin Medical University, Tianjin, China
Mycoplasma pneumoniae (MP) is a common cause of community-acquired pneumonia (CAP), mainly for children and young adults. MP can be implicated in upper respiratory tract infections, bronchiolitis, tracheitis/bronchitis, and pneumonia [1]. Moreover, MP infection has been related to the onset of several extrarespiratory diseases, too [2]. MPrelated extrapulmonary diseases can involve multiple organs, including the joints, muscles, skin, kidneys, heart, gastrointestinal system, nervous system, and hematological system, whereas MP pneumonia is considered to be in part
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