Morphological Characteristics of the Action of Cationic Peptide Warnerin on Regeneration of the Connective Tissue around
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 4, August, 2020
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MORPHOLOGY AND PATHOMORPHOLOGY Morphological Characteristics of the Action of Cationic Peptide Warnerin on Regeneration of the Connective Tissue around Implanted Teflon Catheters in Mice under Conditions of Immunosuppression V. V. Litvinov1, L. M. Lemkina2, G. G. Freund1, and V. P. Korobov2
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 4, pp. 509-513, April, 2020 Original article submitted December 27, 2019 Warnerin pretreatment of catheter segments subcutaneously implanted to mice under conditions of immunosuppression led to a significant increase in the number of neutrophils in the surrounding tissues on day 1; the number of fibroblasts tended to decrease by day 3. Immunohistochemical study showed the presence of T and B lymphocytes on day 3, but no positive reactions to vimentin and CD34 were observed during the first 2 days. These changes suggest that warnerin reduced the intensity of regeneration processes in tissues around the implant, which can be used for suppression of fibrosis. Key Words: modeling of catheter-associated infection; antibacterial peptide warnerin; regeneration; morphology Much recent attention was attracted to new properties of low-molecular-weight antibacterial peptides. In addition to activation of bacterial autolysins and stimulation of lytic destruction of bacterial cells, these compounds have a direct effect on the protective and regenerative mechanisms of the host by promoting migration and differentiation of different cells [7]. Some antibacterial peptides promote sequestration of LPS of gram-negative bacteria [10] and act as potent chemoattractants for antigen-presenting cells, the main components of the adaptive immune response [8]. It was reported that antibacterial peptides of the defensin family stimulated angiogenesis at the sites of their localization and promoted healing of aseptic skin wounds [1]. E. A. Wagner Perm’ State Medical University, Ministry of Health of the Russian Federation; 2Institute of Ecology and Microorganic Genetics, Ural Division of Russian Academy of Sciences, Perm’, Russia. Address for correspondence: [email protected]. V. V. Litvinov 1
Of particular interest are the antibacterial peptides of microorganisms, in particular, warnerin, a low-molecular-weight antibacterial cationic peptide isolated from the culture media of S. warneri IEGM KL-1 bacteria. The peptide producer belongs to the group of coagulase-negative staphylococci, one of the main commensals of the skin and mucosae in humans and animals. Warnerin (molecular weight 2999 Da) is enriched with lysine residues, which determines its pronounced cationic properties and high affinity for negatively charged surface components of bacterial cells [2]. In vitro studies have demonstrated that the bactericidal effect of warnerin is largely determined by dissipation of the membrane potential of the attacked gram-positive bacteria within the first minutes of its interaction with the bacter
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