Nanoparticles with affinity for biopolymer: Bioassisted room-temperature selective multistacking of inorganic particles
- PDF / 346,012 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 46 Downloads / 197 Views
Recently, we selected the antibody fragment with high affinity for the biopolymer film of polyhydroxybutyrate (PHB) from human antibody fragment libraries. In this study, we functionalized CdSe quantum dot (QD) nanoparticles by orderly conjugating the anti-PHB antibody fragments to perform spontaneous and selective stacking of inorganic particles on PHB-coated plates in neutral solutions at room temperature. Surface plasmon resonance analysis showed that the orderly clustering of anti-PHB antibody fragment on QD particles led to no dissociation of QD on PHB-coated plates, indicating the availability of avidity effect. The strong spontaneous immobilization using biomolecular recognition enabled stepwise stacking of inorganic particles on PHB-coated plates only by mixing operation in neutral solutions at room temperature. We show the potential of recombinant anti-material antibody fragments for the bottom-up stacking procedures for hybrid assembly.
I. INTRODUCTION
Downsized inorganic particles can have unique electric and photonic functions, and nanoparticles are applicable for the bottom-up procedures of nanoscale devices. Appropriate patterning and stacking of nanoparticles on substrate can lead the particles to fulfill their potentials. In studies on the patterning and stacking of nanoparticles on substrates, polymer-based materials have been attractive for the development of light flexible devices in the fields of photonics,1 electronics,2 and sensors.3 However, general fabrication processes involve high processing temperature, which causes some damage on polymerbased materials due to lower thermal stability of organic compounds. The techniques of patterning and stacking inorganic nanoparticles at room temperature are potentially useful for the orientation of the nanoparticles in or on heat-labile organic compounds. Recently, several biomimetic approaches have been studied for the assembly and patterning of nano-micro materials at room temperature.4,5 Selective adsorption of inorganic particles on patterned substrates can be controlled by modifying surface functional groups of substrates, and for surface modification of bulk materials,
Address all correspondence to these authors. a) e-mail: [email protected] b) e-mail: [email protected] DOI: 10.1557/JMR.2008.0401 J. Mater. Res., Vol. 23, No. 12, Dec 2008
http://journals.cambridge.org
Downloaded: 02 Feb 2015
the utilization of biomolecules such as deoxyribonucleic acid (DNA), peptides and antibodies is becoming attractive because of their strong selective binding ability. Highly selective base-pairing interactions between complementary single-strand DNA chains have been used for pinpoint deposition of nanoparticles between electrocodes,6 and the peptides with affinity for gold surfaces enabled the patterning of protein and nanoparticles on gold substrates using microcontact printing technology.7,8 Peptides that have the function of molecular recognition with high specificity have been widely utilized in the fields of medical chemistry and
Data Loading...
