Encapsulation of BSA within Gelatin Nanoparticles-laden Biopolymer Film
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Encapsulation of BSA within Gelatin Nanoparticles-laden Biopolymer Film Jin Zhang, Jesse Zhu, Kazi Farida Akhter, and Anu Alice Thomas Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B7, Canada. ABSTRACT This research project aims at the development of new biopolymer nanocomposites which enable to act as a critical component to next generation medical device. In this paper, we developed an in situ method to encapsulate model protein, bovine serum albumin (BSA), within gelatin nanoparticles (NPs). The results demonstrate that the average diameter of the BSA-containing gelatin NPs is approximately 180 nm±10 nm. They can absorb up to 70% of water. The gelatinnanoencapuslated BSA afterwards were loaded in the biopolymer film with thickness around 150 µm composed of poly(2-hydroxyethyl methacrylate) (pHEMA) through the photopolymerization. The release kinetics of BSA from the nanoparticles and the nanoparticleladen p(HEMA) were studied through UV-Vis spectrometry, respectively. The releasing concentration of BSA increased with time (t), and about 80% of the encapsulated BSA was released in 80 hrs; while, the releasing profile of BSA from the gelatin nanoparticles-loaded hydrogel can be monitored up to10 days. These studies show that the gelatin nanoparticles are able to encapsulate water-soluble protein. The hydrogel film, pHEMA, enable to further prolong the releasing profile for the water soluble protein. It is expected that this new biodegradable polymer nanocomposites can be an alternative materials for consisting of implant medical device for protein therapy. INTRODUCTION protein drugs, including insulin, erythropoietin, cytostatic immunosuppressive, and antiinflammatory agents, boosts the protein therapeutics market increasing from $25 billion in 2001 to $51 billion in 2005 [1]. It requests suitable delivery system which is able to not only deliver protein drugs, but also show the biocompatibility. Hydrogels are hydrophilic polymers and have the capacity to absorb large amount of water. They are able to be synthesized, or can be found in nature. Gelatin, a natural polymer, has long been used in food and pharmaceutical industry because of its many advantageous properties, including biodegradability and unique gel-forming ability [2]. Recently, gelatin nanoparticles (NPs) have been used as non-viral vehicle to deliver DNA for gene therapy [3]. To date, very few research have been explored in delivering protein drugs through the gelatin-based nanosystem. Here, we develop an in situ route to encapsulate model protein, bovine serum albumin (BSA), within gelatin nanoparticles through a water soluble crosslinking agent. The nanoencapsulation product, BSA-containing gelatin NPs and gelatin NPs-laden poly(2-hydroxyethyl methacrylate) pHEMA, has been characterized in terms of morphology, particle size, water content, and protein release.
EXPERIMENTS a). Synthesis of BSA Encapsulated Gelatin NPs Gelatin obtained from the Aldrich was dissolved in deionic d
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