NTP4 modulates miRNA accumulation via asymmetric modification of miRNA/miRNA* duplex
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P4 modulates miRNA accumulation via asymmetric modification of miRNA/miRNA* duplex 1,2†
Wenwen Kong 1
3†
1,2
4
1
1
, Xianxin Dong , Yongbing Ren , Yuan Wang , Xintong Xu , Beixin Mo , 1* 1* Yu Yu & Xiaoyan Wang
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Institute of Innovative Biotechnology, College of Life Sciences and 2
Oceanography, Shenzhen University, Shenzhen 518060, China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; Advanced Institute of Natural Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China; 4 State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China 3
Received May 28, 2020; accepted August 21, 2020; published online September 9, 2020
Citation:
Kong, W., Dong, X., Ren, Y., Wang, Y., Xu, X., Mo, B., Yu, Y., and Wang, X. (2020). NTP4 modulates miRNA accumulation via asymmetric modification of miRNA/miRNA* duplex. Sci China Life Sci 63, https://doi.org/10.1007/s11427-020-1803-9
Dear Editor, MicroRNAs (miRNAs) are endogenous small RNAs (sRNAs) that play crucial regulatory roles in gene expression. In plants, the transcribed primary miRNAs (pri-miRNAs) are sequentially processed by DICER-LIKE 1 (DCL1) into precursor miRNAs (pre-miRNAs), and eventually miRNA/miRNA* duplexes (Wang et al., 2019). The methyltransferase HUA ENHANCER 1 (HEN1) confers 2’-Omethylation on the 3’ terminal ribose of each strand within the miRNA/miRNA* duplexes (Yu et al., 2005). Once methylated, miRNA duplexes are loaded onto ARGONAUTE1 (AGO1), with one strand referred to as miRNA which is stabilized to form RNA-induced silencing complexes (RISCs), whereas the other strand becomes miRNA* that is eliminated and degraded. The Arabidopsis genome encodes ten putative noncanonical nucleotidyl transferase proteins (NTPs) that mediate non-templated nucleotide addition to various RNA substrates. HEN1 SUPPRESSOR1 (HESO1/NTP1) and
†Contributed equally to this work *Corresponding authors (Yu Yu, email: [email protected]; Xiaoyan Wang, email: [email protected])
UTP:RNA uridylyltransferase 1 (URT1/NTP3) were the first two identified NTPs that uridylate unmethylated sRNAs and promote their degradation (Ren et al., 2012; Zhao et al., 2012; Tu et al., 2015; Wang et al., 2015). Recently, we reported that widespread cytidylation and uridylation of premiRNAs were catalyzed by HESO1, NTP6 and NTP7 (Song et al., 2019). Besides the above described NTPs, no study on the functions of remaining NTPs has been reported so far. Among the ten putative NTPs encoded by Arabidospsis genome, NTP4 and NTP5 share the highest homology with HESO1 (Figure S1A in Supporting Information). Quantitative real-time PCR (qRT-PCR) results showed a ubiquitous expression of NTP4 during plant development (Figure 1A), while NTP5 can hardly be detected. Our histochemical GUS staining of pNTP4::GUS transgenic plants further confirmed the ubiquit
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