On the First Finding of Barcheek Goby Rhinogobius similis (Gobiidae) in the Russian Far East Waters and on The Results o
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On the First Finding of Barcheek Goby Rhinogobius similis (Gobiidae) in the Russian Far East Waters and on The Results of its COI-Genotyping S. V. Shedko* Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia *e-mail: [email protected] Received December 5, 2019; revised December 27, 2019; accepted January 13, 2020
The capture of barcheek goby Rhinogobius similis in Lake Khasan is reported. The lake is located in the southwest of Primorskii krai (42°26′57″ N; 130°36′31″ E). A brief morphological description is given. A fragment of the mitochondrial COI gene was sequenced and compared with haplotypes from other parts of the species range. Keywords: barcheek goby Rhinogobius similis, range, morphology, COI-barcoding DOI: 10.1134/S0032945220050112
Until now, it was believed that two species of longnosed gobies of g Rhinogobius live in the Russian waters of the Far East: R. lindbergi and R. sowerbyi (Pinchuk, 1992; Vasil’eva, 2007; Bogutskaya et al., 2008; Parin et al., 2014). MATERIALS AND METHODS In the collection of fish sampled by the author with a cast net and hand net on 09–11.10.2019 in Lake Khasan, located in the southwest of Primorskii krai (42°26′57″ N 130°36′31″ E), among others there were four goby (Gobiidae) species: Gymnogobius urotaenia (10 specimens SL 61–69 mm), G. taranetzi (1 specimen SL 45 mm), Tridentiger brevispinis (8 specimens SL 22–48 mm), and barcheek goby Rhinogobius similis1 (4 specimens SL 20.0, 20.5, 45.7 and 48.3 mm). The latter was identified as new for the waters of Russia. In an attempt to reveal the origin of R. similis in the Lake Khasan, a fragment of the mitochondrial gene of the first subunit of cytochrome oxidase I (COI) was sequenced in three individuals. A fragment of the COI gene was obtained by the polymerase chain reaction method using the FishF1 and FishR1 primers (Ward et al., 2005) under the conditions recommended by their developers. Amplicon sequencing was performed in both ends with the same primers using the BigDye Terminator v. 3.1 (Applied Biosystems, United States) kit according to the manufacturer’s protocol. The 1 Unitl
recent revision (Suzuki et al., 2016), this species was known as R. giurinus.
products were dispersed on an automatic laser genetic analyzer Nanofor-05 (Syntol, Russia) from the instrument base of the Federal Scientific Center of Biodiversity, Far East Branch, Russian Academy of Sciences. As a comparative material, we used the COI sequences of barcheek goby obtained in other studies (Kim et al., 2011; Jeon et al., 2012; Chen et al., 2015; Yamasaki et al., 2015; Shen et al., 2019). RESULTS AND DISCUSSION Below is a brief morphological characteristics of the studied specimens of barcheek goby from Lake Khasan (Fig. 1), as well as the results of the performed genotyping. The description was compiled based on the studies of two specimens (male and female) with standard length (SL—from the end of the snout to the base of the caudal fin rays) 45.7 and
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