Mitochondrial genome of the sand goby Acentrogobius caninus ( Acentrogobius , Gobiidae)
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TECHNICAL NOTE
Mitochondrial genome of the sand goby Acentrogobius caninus (Acentrogobius, Gobiidae) Zhi‑gang Tu1,2 · Zhi‑ying Zhao2 · Jing Cui2 · Guang‑can Wu2
Received: 4 January 2017 / Accepted: 3 February 2017 © Springer Science+Business Media Dordrecht 2017
Abstract In this study, we first determined and described the complete mitochondrial genome of Acentrogobius caninus, a member of the Family Gobiidae. The complete mitogenome sequence is 16,616 bp in length containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a putative control region (CR), and a light-strand replication origin (OL). Phylogenetic analysis suggests that A. caninus boasts a close relationship with other two species of the genus Acentrogobius. Our results are expected to provide useful molecular information for species identification and further phylogenetic studies of the Gobiiformes family. Keywords Acentrogobius caninus · Mitochondrion · Genome The sand goby, Acentrogobius caninus, lives in muddy and sandy bottoms of mangrove estuary in South China (Hoese 1986). According to field survey, the damage of mangrove ecosystem caused by water pollution and human activity in recent years has threaten the survival of A. caninus and resulted in a sharp reduction of its population quantity (Wang et al. 2009). However, little genetic information of A. caninus or the genus Acentrogobius is available. The genus Acentrogobius contains 11 species while only two mitochondrial genomes of them have been reported so
* Zhi‑ying Zhao [email protected] 1
Hainan University, Haikou, China
2
Hainan Academy of Ocean and Fisheries Sciences, Haikou, China
far. Jin et al. (2012) reported the mitochondrial genome of A. pflaumii (NC_018064) and Yang et al. (2016) reported the mitochondrial genome of A. chlorostigmatoides (KC196074). In this study, we first determined and described the complete mitochondrial genome of A. caninus. The specimen was collected from Hainan Province, China. The whole genomic DNA data was sequenced using the Illumina MiSeq Platform (Illumina, San Diego, CA) and produced 4,261,772 reads with a size of ~1282 Mbp. We then de novo assembled the complete mitogenome of A. caninu and annotated each one of the protein-coding genes, tRNA genes, and rRNA genes by comparing with published sequences of other vertebrate species using Dogma (http:// dogma.ccbb.utexas.edu/) and finally produced by using Sequin (http://www.ncbi.nlm.nih.gov/). A physical map of the mito-genome was generated by OGDRAW (http:// ogdraw.mpimp-golm.mpg.de/). The complete mitogenome sequence is 16,616 bp in length (Fig. 1). The sequence along with all genes annotated has been deposited in GenBank under the accession number of KY088055. The genomic organization is identical to that of the typical vertebrate mtDNA, including 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a putative control region (CR), and a light-strand replication origin (OL). The overall base composition was 28.9% of A, 26.2% of T, 28.5% of C, and 16.4% of G, with a sli
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