Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro
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Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro Katja J Teerds*1,2, Federica MF van Dissel-Emiliani1, Maria P De Miguel1,3, Mieke de Boer-Brouwer1, Lina M Körting2 and Eddy Rijntjes2 Address: 1Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands, 2Department of Animal Sciences, Human and Animal Physiology Group, Wageningen University, Wageningen, the Netherlands and 3Cell Engineering Laboratory, La Paz Hospital, Madrid, Spain Email: Katja J Teerds* - [email protected]; Federica MF van Dissel-Emiliani - [email protected]; Maria P De Miguel - [email protected]; Mieke de Boer-Brouwer - [email protected]; Lina M Körting - [email protected]; Eddy Rijntjes - [email protected] * Corresponding author
Published: 8 November 2007 Reproductive Biology and Endocrinology 2007, 5:43
doi:10.1186/1477-7827-5-43
Received: 17 July 2007 Accepted: 8 November 2007
This article is available from: http://www.rbej.com/content/5/1/43 © 2007 Teerds et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: The initial steps of stem Leydig cell differentiation into steroid producing progenitor cells are thought to take place independent of luteinizing hormone (LH), under the influence of locally produced factors such as leukaemia inhibitory factor (LIF), platelet derived growth factor A and stem cell factor. For the formation of a normal sized Leydig cell population in the adult testis, the presence of LH appears to be essential. Oncostatin M (OSM) is a multifunctional cytokine and member of the interleukin (IL)-6 family that also includes other cytokines such as LIF. In the rat OSM is highly expressed in the late fetal and neonatal testis, and may thus be a candidate factor involved in Leydig cell progenitor formation. Methods: Interstitial cells were isolated from 13-day-old rat testes and cultured for 1, 3 or 8 days in the presence of different doses of OSM (range: 0.01 to 10 ng/ml) alone or in combination with LH (1 ng/ml). The effects of OSM and LH on cell proliferation were determined by incubating the cultures with [3H]thymidine or bromodeoxyuridine (BrdU). Developing progenitor cells were identified histochemically by the presence of the marker enzyme 3beta-hydroxysteroid dehydrogenase (3beta-HSD). Results: OSM, when added at a dose of 10 ng/ml, caused a nearly 2-fold increase in the percentage of Leydig cell progenitors after 8 days of culture. Immunohistochemical double labelling experiments with 3beta-HSD and BrdU antibodies showed that this increase was the result of differentiation of stem Leydig cells/precursor cells and not caused by proliferation of progenitor cells themse
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