One-pot solvothermal method to prepare functionalized Fe 3 O 4 nanoparticles for bioseparation
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Fengxiang Qie,c) Jianxuan Hou, and Shizhong Luo Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China
Liang Luo and Xiaoming Suna) State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China
Tianwei Tanb) Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China (Received 19 September 2011; accepted 23 January 2012)
Surface-functionalized magnetic nanoparticles were prepared by a facile one-pot solvothermal method in ethylene glycol solution. Zeta value, size, and magnetic properties could be well tuned by introducing different functional group molecules. Characterizations, including transmission electronic microscopy, scanning electronic microscopy, thermogravimetric analysis, x-ray powder diffraction and vibrating sample magnetometer, and Fourier transform infrared spectrophotometer demonstrated the efficiency of this simple and general synthesis strategy. The hydrophilic magnetic nanoparticles with various surface functional groups and zeta values were evidenced as excellent candidates for bioseparation by extracting DNA molecules from a model mixture of cell fractures. I. INTRODUCTION
In recent years, magnetic iron oxide nanoparticles have received considerable attention for their superparamagnetic properties and low toxicity. Hydrophilic superparamagnetic iron oxide nanoparticles (HSPIONs) have attracted especial attention on account of their potential superiority for applications, such as bioseparations,1–5 drug delivery,6–9 cell imaging,10–12 and magnetic resonance imaging (MRI).13,14 To date, HSPIONs could be formed by many technologies, such as coprecipitation,15–17 microemulsion method,18 sol–gel method,19 thermal decomposition,20,21 and solvothermal method.22–25 The typical strategy involves iron oxide nanoparticles formation26 and consequent postsynthesis surface modification or polymer encapsulation15,27–29 to make the particles stable in aqueous phase and biocompatible.22,24,30,31 Requirement of expensive and toxic initial materials, rigorous reaction conditions, low yield, time-consuming, and cost inefficiency might limit the scaling-up and applications of HSPIONs.
Very recently, Li’s group made a breakthrough in this field by using ethylene glycol (EG) as both solvent and reducing agent for solvothermal synthesis of HSPION.25 Morphology and crystallization of iron oxide nanomaterials could be easily tuned by this method.32–36 More importantly, the method provided potentials for combining synthesis and surface functionalization in one step. For instance, amine modification was realized and used for detection and purification of biomolecules.2,3,37–39 Potential applications include MRI,10–14 drug carrier,6–9 sensor,40–42 and composite materials.43 Herein, we further developed the method by introducing various tuning reagent
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