Optical nanoscopy
- PDF / 11,251,848 Bytes
- 71 Pages / 439.37 x 666.142 pts Page_size
- 10 Downloads / 167 Views
Optical nanoscopy A. Diaspro1,2
· P. Bianchini1
Received: 31 May 2020 / Accepted: 20 July 2020 © The Author(s) 2020
Abstract This article deals with the developments of optical microscopy towards nanoscopy. Basic concepts of the methods implemented to obtain spatial super-resolution are described, along with concepts related to the study of biological systems at the molecular level. Fluorescence as a mechanism of contrast and spatial resolution will be the starting point to developing a multi-messenger optical microscope tunable down to the nanoscale in living systems. Moreover, the integration of optical nanoscopy with scanning probe microscopy and the charming possibility of using artificial intelligence approaches will be shortly outlined. Keywords Super-resolution · Nanoscopy · Fluorescence · Confocal · Two-photon excitation STED
Contents 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1 Photobleaching . . . . . . . . . . . . . . . . . . . . . . . . . 2.2 Two-photon excitation fluorescence . . . . . . . . . . . . . . . 2.3 Fluorescence resonant energy transfer (FRET) . . . . . . . . . 3 Spatial resolution . . . . . . . . . . . . . . . . . . . . . . . . . . . 3.1 Fourier ring correlation . . . . . . . . . . . . . . . . . . . . . 4 Advanced optical microscopy . . . . . . . . . . . . . . . . . . . . 4.1 Three-dimensional optical sectioning . . . . . . . . . . . . . . 4.2 Confocal laser scanning microscopy . . . . . . . . . . . . . . 4.3 Two-photon excitation microscopy . . . . . . . . . . . . . . . 5 Optical nanoscopy . . . . . . . . . . . . . . . . . . . . . . . . . . 5.1 Coordinate stochastic super-resolved fluorescence microscopy 5.2 Coordinate-targeted super-resolved fluorescence microscopy. . 5.3 Structured Illumination Microscopy . . . . . . . . . . . . . . 5.4 Expansion microscopy . . . . . . . . . . . . . . . . . . . . .
B
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . .
A. Diaspro [email protected]
1
Nanoscopy, CHT, Istituto Italiano di Tecnologia, Genova, Italy
2
DIFILAB, Dipartimento di Fisica, Università degli Studi di Genova, Genova, Italy
123
A. Diaspro, P. Bianchini 6 Correlative nanoscopy . . . . . . 6.1 Atomic force microscopy . . 6.2 Coupling forces and photons 6.3 Nanomanipulation . . . . . . 7 Conclusions . . . . . . . . . . . References . . . . . . . . . . . . . .
. . . . . .
. . . . . .
. . . . . .
. . . . . .
. . . . . .
. . . . . .
Data Loading...
