Osteopontin-4 and Osteopontin-5 splice variants are expressed in several tumor cell lines
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SHORT COMMUNICATION
Osteopontin‑4 and Osteopontin‑5 splice variants are expressed in several tumor cell lines Gabriela Ribeiro Silva1,5 · Daniella Santos Mattos1,2 · Ana Clara Fonseca Bastos1,2 · Bruna Prunes Pena Baroni Viana1,2 · Mariana Concentino Menezes Brum1,2 · Luciana Bueno Ferreira1,2 · Etel Rodrigues Pereira Gimba1,2,3,4,5 Received: 11 May 2020 / Accepted: 25 September 2020 © Springer Nature B.V. 2020
Abstract Among osteopontin splice variants (OPN-SV), the expression profile of osteopontin-4 (OPN4) and osteopontin-5 (OPN5) has not been addressed in distinct cancer types. We herein aimed to investigate their expression in several cancer cell lines, besides comparing it in relation to the three previously described OPN-SV: OPNa, OPNb and OPNc. Total RNA from cancer cell lines, including prostate (PC3 and DU145), ovarian (A2780), breast (MCF-7 and MDA-MB-231), colorectal (Caco-2, HT-29 and HCT-116), thyroid (TT, TPC1 and 8505c) and lung (A549 and NCI-H460) was extracted, followed by cDNA synthesis. OPN-SV transcript analysis by RT-PCR or RT-qPCR were performed using OPN-SV specific oligonucleotides and gapdh and actin transcripts were used as housekeeping controls. OPN4 and OPN5 transcripts displayed co-expression in most tested cell lines. OPN4 was found expressed in similar or higher levels in relation to OPN5. Moreover, in most tested cell lines, OPN4 is also expressed in similar levels to OPNa or OPNb. The expression of OPN5 is also generally variable in relation to the other OPN-SV, but expressed in similar or higher levels in relation to OPNc, depending on each tested cell line. OPN4 and OPN5 seem to be co-expressed in several tumor types and OPN4 is one of the most overexpressed OPNSV in distinct tumor cell lines. Once both OPN4 and OPN5 are differentially expressed and also evidence tumor-specific expression patterns, we hypothesize that similarly to the other OPN-SV, they also possibly contribute to key aspects of tumor progression, what should be further functionally investigated in distinct tumor models. Keywords Osteopontin · Splice variants · Splicing · OPN4 · OPN5 · Cancer cell lines
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05867-9) contains supplementary material, which is available to authorized users. * Etel Rodrigues Pereira Gimba [email protected] Gabriela Ribeiro Silva [email protected] Daniella Santos Mattos [email protected] Ana Clara Fonseca Bastos [email protected] Bruna Prunes Pena Baroni Viana [email protected]
1
Programa de Oncobiologia Celular e Molecular, Instituto Nacional de Câncer, Rua André Cavalcanti, 37, 3° andar, Rio de Janeiro CEP: 20 231 050, Brazil
2
Programa de Pós‑Graduação Stricto Sensu em Oncologia, Instituto Nacional de Câncer, Rua André Cavalcanti, 37, 3° andar, Rio de Janeiro CEP: 20 231 050, Brazil
3
Divisão de Pesquisa Clínica, Instituto Nacional de Câncer, Rua André Cavalcanti, 37, 3° andar, Rio de Janeiro CEP: 20 231
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